CpG motif-based adjuvant as a replacement for Freund's complete adjuvant in a recombinant LHRH vaccine

被引：19
|
作者：
Conforti, Valeria A. [1 ]
De Avila, David M. [1 ]
Curnmings, Nada S. [1 ]
Zanella, Ricardo [1 ]
Wells, Kenny J. [1 ]
Uelker, Hasan [2 ]
Reeves, Jerry J. [1 ]
机构：
[1] Washington State Univ, Dept Anim Sci, Pullman, WA 99164 USA
[2] Yuzuncu Yil Univ, Ziraat Fac, Dept Anim Sci, TR-65080 Van, Turkey
来源：
VACCINE | 2008年 / 26卷 / 07期
关键词：
LHRH immunization; CpG ODN; Freund's adjuvant;
D O I：
10.1016/j.vaccine.2007.12.010
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
This study compared: (1) Freund's complete adjuvant and CpG oligodeoxynucleotide (ODN) 2006 in water-in-oil emulsion as adjuvants; and (2) increasing doses of a recombinant ovalbumin-LHRH (ova-LHRH) fusion protein as an antigen for a contraceptive vaccine. Treatment groups (n = 8 heifers/group) were: one untreated control group; five groups receiving CpG ODN with different doses of ova-LHRH (1.5; 2.3; 3.4; 5.1; and 7.6 mg); and one group receiving 3.4 mg ova-LHRH in Freund's. Heifers were immunized at weeks 0 and 14. Ail vaccine treatments caused gonadal regression and estrus suppression. CpG ODN is a suitable replacement for Freund's for LHRH immunization. (c) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码：907 / 913
页数：7
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