Development of sensitive and fast immunoassays for amantadine detection

被引：44

作者：

Xu, Liguang ^{[1
,2
]}

Peng, Shuang ^{[1
,2
]}

Liu, Liqiang ^{[1
,2
]}

Song, Shanshan ^{[1
,2
]}

Kuang, Hua ^{[1
,2
]}

Xu, Chuanlai ^{[1
,2
]}

机构：

[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

[2] Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

来源：

FOOD AND AGRICULTURAL IMMUNOLOGY | 2016年 / 27卷 / 05期

关键词：

Amantadine; chicken samples; ic-ELISA; immunochromatographic strip; IMMUNOCHROMATOGRAPHIC ASSAY; PRECOLUMN DERIVATIZATION; STRIP TEST; ANTIBODY; PLASMA;

D O I：

10.1080/09540105.2016.1148667

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A sensitive indirect competitive enzyme-linked immunosorbent assay(ic-ELISA) method and a gold nanoparticle immunochromatographic strip were developed to detect amantadine (AM) in foods. A novel AM hapten was prepared, and a sensitive monoclonal antibody against AM was developed. The optimum ic-ELISA conditions included a pH of 7.4 and an ionic strength of 1.6% with no organic solvents. The half-inhibition concentration (IC50) value of ic-ELISA was 1.92ng/ml, with a limit of detection of 0.62ng/ml. The immunochromatographic test strip method had a visual cut-off value at 5g/kg. Chicken samples were spiked with three concentrations of AM (1, 2, and 5 mu g/kg) and analysed by ic-ELISA. Good recoveries were obtained (92.3% at 1 mu g/kg AM, 116.1% at 2 mu g/kg AM, and 91.8% at 5 mu g/kg AM).

引用

页码：678 / 688

页数：11

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