Development of sensitive and fast immunoassays for amantadine detection

被引：44
|
作者：
Xu, Liguang [1 ,2 ]
Peng, Shuang [1 ,2 ]
Liu, Liqiang [1 ,2 ]
Song, Shanshan [1 ,2 ]
Kuang, Hua [1 ,2 ]
Xu, Chuanlai [1 ,2 ]
机构：
[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China
[2] Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China
来源：
FOOD AND AGRICULTURAL IMMUNOLOGY | 2016年 / 27卷 / 05期
关键词：
Amantadine; chicken samples; ic-ELISA; immunochromatographic strip; IMMUNOCHROMATOGRAPHIC ASSAY; PRECOLUMN DERIVATIZATION; STRIP TEST; ANTIBODY; PLASMA;
D O I：
10.1080/09540105.2016.1148667
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A sensitive indirect competitive enzyme-linked immunosorbent assay(ic-ELISA) method and a gold nanoparticle immunochromatographic strip were developed to detect amantadine (AM) in foods. A novel AM hapten was prepared, and a sensitive monoclonal antibody against AM was developed. The optimum ic-ELISA conditions included a pH of 7.4 and an ionic strength of 1.6% with no organic solvents. The half-inhibition concentration (IC50) value of ic-ELISA was 1.92ng/ml, with a limit of detection of 0.62ng/ml. The immunochromatographic test strip method had a visual cut-off value at 5g/kg. Chicken samples were spiked with three concentrations of AM (1, 2, and 5 mu g/kg) and analysed by ic-ELISA. Good recoveries were obtained (92.3% at 1 mu g/kg AM, 116.1% at 2 mu g/kg AM, and 91.8% at 5 mu g/kg AM).
引用
收藏
页码：678 / 688
页数：11
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