Effect of oxygen supply on Monascus pigments and citrinin production in submerged fermentation

被引:44
|
作者
Yang, Jian [1 ]
Chen, Qi [1 ]
Wang, Weiping [1 ]
Hu, Jiajun [1 ]
Hu, Chuan [1 ]
机构
[1] Hubei Univ Technol, Hubei Prov Cooperat Innovat Ctr Ind Fermentat, Key Lab Fermentat Engn, Minist Educ, Wuhan 430068, Peoples R China
基金
中国国家自然科学基金;
关键词
Monascus; Oxygen supply; Citrinin; Pigments; Submerged fermentation; RED PIGMENT; PURPUREUS; GROWTH; ENHANCEMENT; CULTIVATION; CULTURE; ACID;
D O I
10.1016/j.jbiosc.2014.10.014
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The influence of oxygen supply on Monascus pigments and citrinin production by Monascus ruber HSA000 in submerged fermentation was studied. For Monascus cultivation with high pigments and low citrinin production, the initial growth phase, mid-stage phase, and later-stage production phase were separated by shifting oxygen supply. The optimal condition for the fermentation process in shake-flask fermentation was a three-stage rotating rate controlled strategy (0-48 h at 150 rpm, 48-108 h at 250 rpm, 108-120 h at 200 rpm) with medium volume of 100 mL added to 250 mL Erlenmeyer flasks at 30 degrees C for 120 h cultivation. Compared to constant one-stage cultivation (medium volume of 100 mL, rotating rate of 250 rpm), the pigments were reduced by 40.4%, but citrinin was reduced by 64.2%. The most appropriate condition for the fermentation process in a 10 L fermentor is also a three-stage aeration process (0-48 h at 300 L/h, 48-96 h at 500 L/h, 96-120 h at 200 L/h) with agitation of 300 rpm at 30 degrees C for 120 h cultivation, and 237.3 +/- 5.7 U/mL pigments were produced in 120 h with 6.05 +/- 0.19 mg/L citrinin in a 10 L fermentor. Compared to aeration-constant (500 L/h) cultivation, pigment production was increased by 29.6% and citrinin concentration was reduced by 79.5%. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.
引用
收藏
页码:564 / 569
页数:6
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