Simultaneous Determination of Methotrexate and Sulphasalazine in Plasma by HPLC-DAD

被引：0
|
作者：
Joseph, Siji [1 ]
Menon, Sreelakshmy [1 ]
Khera, Smriti [2 ]
机构：
[1] Agilent Technol India Pvt Ltd, Life Sci Ctr, Bangalore, Karnataka, India
[2] Agilent Technol, Life Sci & Diagnost Grp, Santa Clara, CA 95051 USA
来源：
LC GC EUROPE | 2015年 / 28卷 / 05期
关键词：
RHEUMATOID-ARTHRITIS; COMBINATION THERAPY; DOUBLE-BLIND; EFFICACY;
D O I：
暂无
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Here we describe a simple and robust high performance liquid chromatography-diode array detection (HPLC-DAD) method for the simultaneous determination of methotrexate (MTX) and sulphasalazine (SSZ) from plasma. MTX and SSZ are used in combination for the treatment of rheumatoid arthritis. Using two detector wavelengths, 304 nm for MTX and 358 nm for SSZ, we were able to selectively quantitate both analytes during the same chromatographic run. The method was validated using quality control samples for critical analytical performance criteria of recovery, reproducibility, selectivity, accuracy, and precision.
引用
收藏
页码：272 / +
页数：5
相关论文
共 50 条
  • [41] Determination of ethylene thiourea in urine by HPLC-DAD
    El Balkhi, S
    Sandouk, P
    Gailiot-Guilley, M
    JOURNAL OF ANALYTICAL TOXICOLOGY, 2005, 29 (04) : 229 - 233
  • [42] Determination of Capsinoids by HPLC-DAD in Capsicum Species
    Singh, Satyavan
    Jarret, Robert
    Russo, Vincent
    Majetich, George
    Shimkus, Joel
    Bushway, Rodney
    Perkins, Brian
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2009, 57 (09) : 3452 - 3457
  • [43] Simultaneous Determination of Eight Components in Leonuri Herba from Different Habitats by HPLC-DAD
    Xu, Yang
    Yang, Huailei
    Chi, Jihong
    Wang, Fang
    Bao, Huiwei
    JOURNAL OF CHROMATOGRAPHIC SCIENCE, 2020, 58 (05) : 464 - 470
  • [44] Simultaneous determination of cucurbitacins B, D, and E in Cucurbita seed oils by HPLC-DAD
    Goya-Jorge, Elizabeth
    Jorge-Rodriguez, Maria Elisa
    Saucedo-Hernandez, Yanelis
    NATURAL PRODUCT RESEARCH, 2024,
  • [45] Simultaneous determination of six active components in traditional herbal medicine 'Oyaksungisan' by HPLC-DAD
    Weon, Jin Bae
    Yang, Hye Jin
    Ma, Jin Yeul
    Ma, Choong Je
    JOURNAL OF NATURAL MEDICINES, 2012, 66 (03) : 510 - 515
  • [46] Simultaneous Determination of Five Active Constituents in the Aerial Parts of Saururus chinensis by HPLC-DAD
    Kim, Seung Hyun
    Choi, Eun Jung
    Lee, Mi Kyeong
    Lee, Ki Yong
    Park, Jung Hyun
    Kim, Young Choong
    Sung, Sang Hyun
    JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2009, 32 (20) : 2943 - 2953
  • [47] Simultaneous determination of phenytoin and dextromethorphan in urine by solid-phase extraction and HPLC-DAD
    Santagati, NA
    Gotti, R
    Ronsisvalle, G
    JOURNAL OF SEPARATION SCIENCE, 2005, 28 (11) : 1157 - 1162
  • [48] Simultaneous Determination of Multiple Bioactive Constituents in Total Alkaloid of Sophora alopecuroides by HPLC-DAD
    ZHAO Wen-chang
    Chinese Herbal Medicines, 2011, (01) : 66 - 69
  • [49] DETERMINATION OF PESTICIDES IN WINES SAMPLES BY HPLC-DAD AND HPTLC-DAD
    Tuzimski, Tomasz
    JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2012, 35 (10) : 1415 - 1428
  • [50] Simultaneous HPLC-DAD Determination of Retinol and Eight Vitamin E Isomers in Human Serum
    Yang, Yi
    Lu, Dan
    Zhang, Jing
    Li, Yongxin
    Zheng, Bo
    Sun, Chengjun
    CHROMATOGRAPHIA, 2015, 78 (21-22) : 1359 - 1366
12345