A feasibility study of recombinant adeno-associated virus as a vector for transferring a target gene to retina

被引：0
|
作者：
Wang, Jian-Ming [1 ]
Fan, Ya-Zhi [1 ]
Hui, Na [1 ]
Xiong, Lei [1 ]
Feng, Hai-Xiao [1 ]
Sun, Nai-Xue [1 ]
机构：
[1] Xi An Jiao Tong Univ, Affiliated Hosp 2, Coll Med, Dept Ophthalmol, Xian 710004, Shaanxi, Peoples R China
来源：
INTERNATIONAL JOURNAL OF OPHTHALMOLOGY | 2009年 / 2卷 / 02期
关键词：
recombinant adeno-associated virus; green fluorescent protein gene; gene transfer; retina; TRANSGENE EXPRESSION; DELIVERY;
D O I：
暂无
中图分类号：
R77 [眼科学];
学科分类号：
100212 ;
摘要：
AIM: To study the feasibility of recombinant adeno-associated virus (rAAV) as a vector to transfer the green fluorescent protein (GP) gene as a target gene into rabbit retina. METHODS: Intravitreal injection of rAAV-gfp was performed in either eye for each rabbit with the other eye taken as control. At the 3rd, 7th, and 14th day after injection, the eyeballs were removed, and the retinas were flat-mounted on glass slides to inspect the retinal fluorescence, respectively. RESULTS: After intravitreal injection of rAAV-gfp, the presence of fluorescent spots in the cytoplasm of retinal cells indicated that GFP gene was efficiently transferred and expressed in the rabbit retina. CONCLUSION: Recombinant adeno-assodated virus is a reliable and simple vector for transferring target gene, e.g., GFP gene, to the retina.
引用
收藏
页码：143 / 145
页数：3
相关论文
共 50 条
  • [41] Molecular design for recombinant adeno-associated virus (rAAV) vector production
    Aponte-Ubillus, Juan Jose
    Barajas, Daniel
    Peltier, Joseph
    Bardliving, Cameron
    Shamlou, Parviz
    Gold, Daniel
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2018, 102 (03) : 1045 - 1054
  • [42] Genetic fate of recombinant adeno-associated virus vector genomes in muscle
    Schnepp, BC
    Clark, KR
    Klemanski, DL
    Pacak, CA
    Johnson, PR
    JOURNAL OF VIROLOGY, 2003, 77 (06) : 3495 - 3504
  • [43] Recombinant adeno-associated virus vector-based gene transfer for defects in oxidative metabolism
    Owen, R
    Lewin, AP
    Peel, A
    Wang, JM
    Guy, J
    Hauswirth, WW
    Stacpoole, PW
    Flotte, TR
    HUMAN GENE THERAPY, 2000, 11 (15) : 2067 - 2078
  • [44] Adeno-associated virus vector as a platform for gene therapy delivery
    Dan Wang
    Phillip W. L. Tai
    Guangping Gao
    Nature Reviews Drug Discovery, 2019, 18 : 358 - 378
  • [45] Intravitreal transduction profile of recombinant adeno-associated virus in murine and human retina
    Ertel, Kristina
    Reid, Chris
    Lipinski, Daniel M.
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2017, 58 (08)
  • [46] Hot topics in adeno-associated virus as a gene transfer vector
    Zhao, N
    Liu, DP
    Liang, CC
    MOLECULAR BIOTECHNOLOGY, 2001, 19 (03) : 229 - 237
  • [47] Hot topics in adeno-associated virus as a gene transfer vector
    Na Zhao
    De-Pei Liu
    Chih-Chuan Liang
    Molecular Biotechnology, 2001, 19 : 229 - 237
  • [48] Adeno-associated virus vector integration
    Deyle, David R.
    Russell, David W.
    CURRENT OPINION IN MOLECULAR THERAPEUTICS, 2009, 11 (04) : 442 - 447
  • [49] Adeno-associated virus vector as a platform for gene therapy delivery
    Wang, Dan
    Tai, Phillip W. L.
    Gao, Guangping
    NATURE REVIEWS DRUG DISCOVERY, 2019, 18 (05) : 358 - 378
  • [50] Adeno-associated virus as a gene delivery vector for liver cells
    Lau, JYN
    JOURNAL OF HEPATOLOGY, 1999, 31 (02) : 385 - 385
12345