Isoflavones as Apoptosis Inducers in Human Hepatoma HuH-7 Cells

被引：54

作者：

Mansoor, Tayyab A. ^{[1
]}

Ramalho, Rita M. ^{[1
]}

Luo, Xuan ^{[1
]}

Ramalhete, Catia ^{[1
]}

Rodrigues, Cecilia M. P. ^{[1
]}

Ferreira, Maria-Jose U. ^{[1
]}

机构：

[1] Univ Lisbon, Fac Pharm, Res Inst Med & Pharmaceut Sci, P-1649003 Lisbon, Portugal

来源：

PHYTOTHERAPY RESEARCH | 2011年 / 25卷 / 12期

关键词：

Pycnanthus angolensis; Myristicaceae; apoptosis induction; HuH-7; cells; isoflavones; flavonoids; PYCNANTHUS-ANGOLENSIS; CANCER-CELLS; CYTOTOXIC CONSTITUENTS; MULTIDRUG-RESISTANCE; INDUCTION; MODULATION; GENISTEIN; LIGNANS; THERAPY; POTENT;

D O I：

10.1002/ptr.3498

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Nine flavonoids isolated from the ethyl acetate extract of Pycnanthus angolensis were assayed for their potential apoptosis induction activities in human hepatoma HuH-7 cells. These flavonoids include eight isoflavones, namely irilone (1), tectorigenine (2), formononetin (3), genistein (4), 2'-hydroxybiochanin A (5), mixture of biochanin A (6) and prunetin (7), and 4',7-dihydroxy-2'-methoxyisoflavan (8), and the flavanone liguiritigentin (9). Their chemical structures were characterized by spectroscopic methods including 2D NMR experiments. Methodology for cell death detection included the LDH assay, Hoechst staining, TUNEL staining and general caspase-3-like activity assay. The compounds tested showed higher apoptosis induction profiles in HuH-7 cells compared with the control. Caspase activity assays confirmed the apoptosis inducing activity of these flavonoids. Copyright (C) 2011 John Wiley & Sons, Ltd.

引用

页码：1819 / 1824

页数：6

共 50 条

[31] Huh-7: A Human "Hemochromatotic" Cell Line
Vecchi, Chiara
Montosi, Giuliana
Pietrangelo, Antonello
HEPATOLOGY, 2010, 51 (02) : 654 - 659
[32] Effects of cytokines, butyrate and dexamethasone on serum amyloid a and apolipoprotein A-I synthesis in human HUH-7 hepatoma cells
Malle, E
Leonhard, B
Knipping, G
Sattler, W
SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 1999, 50 (02) : 183 - 187
[33] Evaluation of CYP3A4 inhibition and hepatotoxicity using DMSO-treated human hepatoma HuH-7 cells
Liu, Yitong
Flynn, Thomas J.
Xia, Menghang
Wiesenfeld, Paddy L.
Ferguson, Martine S.
CELL BIOLOGY AND TOXICOLOGY, 2015, 31 (4-5) : 221 - 230
[34] Enhanced phosphorylation and sulfation of human recombinant factor IX for production by the hepatoma cell line HuH-7
Enjolras, N.
Dargaud, Y.
Perot, E.
Guillaume, F.
Becchi, M.
Negrier, C.
HAEMOPHILIA, 2012, 18 : 28 - 28
[35] Suppression of albumin and α-fetoprotein gene expression by butyrolactone I, a selective inhibitor of the cdk family, in HuH-7 human hepatoma cells
Hida, D
Nakata, K
Shima, Y
Migita, K
Nakao, K
Kato, Y
Ishii, N
Eguchi, K
ANTICANCER RESEARCH, 1998, 18 (6A) : 4317 - 4322
[36] Metformin induces apoptosis in hepatocellular carcinoma Huh-7 cells in vitro and its mechanism
林芬
China Medical Abstracts(Internal Medicine), 2013, 30 (04) : 225 - 225
[37] Enhanced Glucose Requirement in Human Hepatoma-derived HuH-7 Cells by Forced Expression of the bcl-2 Gene
Okamoto, Kyoko
Muraguchi, Takashi
Shidoji, Yoshihiro
JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION, 2008, 43 (02) : 101 - 108
[38] Evaluation of CYP3A4 inhibition and hepatotoxicity using DMSO-treated human hepatoma HuH-7 cells
Yitong Liu
Thomas J. Flynn
Menghang Xia
Paddy L. Wiesenfeld
Martine S. Ferguson
Cell Biology and Toxicology, 2015, 31 : 221 - 230
[39] Induction of filamin-C and its involvement in the regulation of cellular senescence and apoptosis in Huh-7 hepatoma cells during arsenic trioxide exposure
Aki, Toshihiko
Kukita, Mitsuki
Takata, Mao
Funakoshi, Takeshi
Unuma, Kana
Uemura, Koichi
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2023, 651 : 92 - 97
[40] Intracellularly transported adenosine induces apoptosis in HuH-7 human hepatoma cells by downregulating c-FLIP expression causing caspase-3/-8 activation
Yang, Dongqin
Yaguchi, Takahiro
Yamamoto, Hideyuki
Nishizaki, Tomoyuki
BIOCHEMICAL PHARMACOLOGY, 2007, 73 (10) : 1665 - 1675

← 1 2 3 4 5 →