DNA binding and nuclease activity of copper(II) complexes of tridentate ligands

被引：66
|
作者：
Kumar, Pankaj [1 ]
Baidya, Basudeb [1 ]
Chaturvedi, Sumit Kumar [2 ]
Khan, Rizwan Hasan [2 ]
Manna, Debasis [1 ]
Mondal, Biplab [1 ]
机构：
[1] Indian Inst Technol Guwahati, Dept Chem, N Guwahati 781039, Assam, India
[2] Aligarh Muslim Univ, Interdisciplinary Biotechnol Unit, Aligarh 202002, Uttar Pradesh, India
来源：
INORGANICA CHIMICA ACTA | 2011年 / 376卷 / 01期
关键词：
Copper complexes; DNA; Binding; Cleavage; VITRO ANTITUMOR-ACTIVITY; CLEAVAGE ACTIVITY; STRAND SCISSION; CRYSTAL-STRUCTURES; PLASMID DNA; EFFICIENT; HYDROLYSIS; ACIDS; INTERCALATOR; RECOGNITION;
D O I：
10.1016/j.ica.2011.06.022
中图分类号：
O61 [无机化学];
学科分类号：
070301 ; 081704 ;
摘要：
Two copper(II) complexes, 1 and 2 with L-1 and L-2 [L-1 = 2-hydroxybenzyl(2-(pyridin-2-yl)ethylamine); L-2 = 2-hydroxybenzyl(2-(pyridin-2-yl)methylamine)] ligands, respectively, have been synthesized and characterized. The interaction of both the complexes with DNA has been studied to explore their potential biological activity. The DNA binding properties of the complexes with calf thymus (CT) DNA were studied by spectroscopic titration. The complexes show binding affinity to CT DNA with binding constant (K-b) values in the order of 10(5) M-1. Thermal denaturation and circular dichroism studies suggest groove binding of the complexes to CT DNA. Complexes also exhibit strong DNA cleavage activity in presence of reducing agents like 3-mercaptopropionic acid and beta-mercaptoethanol. Mechanistic studies reveal the involvement of reactive hydroxyl radicals for their DNA cleavage activity. (C) 2011 Elsevier B. V. All rights reserved.
引用
收藏
页码：264 / 270
页数：7
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