Rice powder waste enzymatic hydrolysis for production of second generation ethanol

The objective of the present investigation was to hydrolyze the rice powder to reducing sugars and, through alcoholic fermentation, obtain bioethanol. Nine solutions of ground rice powder (8%, 13% and 18%) and purified water were prepared and inoculated with conidia of Trichoderma spp in concentrations (0.2 g / L, 0.4 g / L and 0.6 g). / L), which were incubated at 30 degrees C for 144 hours. Using the DNA method (3, 5 di nitrosalisilic acid), the reducing sugars were quantified: treatment A: 4.32 +/- 0.08%, B: 5.27 +/- 0.07%, C: 5.10 +/- 0, 01%, D: 5.53 +/- 0.12%, E: 8.24 +/- 0.06%, F: 6.37 +/- 0.07%, G: 5.13 +/- 0.02%, H: 7, 19 +/- 0.26%, I: 9.69 +/- 0.18% and ethanol by gas chromatography. The treatment with the highest percentage of bioconversion was treatment I (18% and 0.6 g / L inoculum), where the glucose concentration was 9.88%, and the ethanol yield was 5 degrees GL. In conclusion it is possible to hydrolyze the total carbohydrates present in the rice powder bz reducing sugars and the subsequent alcoholic fermentation.