A multilocus variable number tandem repeat analysis assay provides high discrimination for genotyping Leptospira santarosai strains

被引：22
|
作者：
Hamond, Camila [1 ]
Pinna, Melissa [2 ]
Medeiros, Marco Alberto [3 ]
Bourhy, Pascale [4 ,5 ]
Lilenbaum, Walter [1 ]
Picardeau, Mathieu [4 ,5 ]
机构：
[1] Univ Fed Fluminense, Lab Vet Bacteriol, Dept Microbiol & Parasitol, Niteroi, RJ, Brazil
[2] Univ Fed Bahia, Dept Prevent Vet Med & Anim Prod, Salvador, BA, Brazil
[3] Brazilian Minist Hlth, Biomanguinhos, Oswaldo Cruz Fdn, Rio De Janeiro, RJ, Brazil
[4] Inst Pasteur, Unite Biol Spirochetes, Natl Reference Ctr, Paris, France
[5] WHO Collaborating Ctr Leptospirosis, Paris, France
来源：
JOURNAL OF MEDICAL MICROBIOLOGY | 2015年 / 64卷
基金：
美国国家卫生研究院;
关键词：
MOLECULAR CHARACTERIZATION; SEROVAR; CATTLE; BRAZIL; INTERROGANS; SPP; PCR;
D O I：
10.1099/jmm.0.000045
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Considering the prevalence of Leptospira santarosai infections in the Americas and the scarce information about the species, we aimed to apply a multilocus variable number tandem repeat (VNTR) analysis (MLVA) for the molecular typing of L. santarosai isolates from various sources. Amplification of three VNTR loci selected from L. santarosaigenome sequences resulted in a wide range of sizes for the amplified products amongst the 21 L. santarosai strains analysed. This suggested a variation in tandem repeat copy numbers in the VNTR loci. secY sequencing also showed a high nucleotide diversity, confirming the MLVA data. In conclusion, this novel MLVA provided a high level of discrimination between L. santarosai isolates, and this new typing tool could be used to investigate leptospirosis in regions where L. santarosai predominates.
引用
收藏
页码：507 / 512
页数：6
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