Isolation and functional characterization of proinflammatory acidic phospholipase A2 from Bothrops leucurus snake venom

被引：32

作者：

Nunes, Debora C. O. ^{[1
]}

Rodrigues, Renata S. ^{[2
]}

Lucena, Malson N. ^{[3
]}

Cologna, Camila T. ^{[2
]}

Oliveira, Ana Carolina S. ^{[4
]}

Hamaguchi, Amelia ^{[1
]}

Homsi-Brandeburgo, Maria I. ^{[1
]}

Arantes, Eliane C. ^{[2
]}

Teixeira, David N. S. ^{[4
]}

Ueira-Vieira, Carlos ^{[1
]}

Rodrigues, Veridiana M. ^{[1
,5
]}

机构：

[1] Univ Fed Uberlandia, Inst Genet & Bioquim, BR-38400 Uberlandia, MG, Brazil

[2] Univ Sao Paulo, Fac Ciencias Farmaceut, BR-14049 Ribeirao Preto, RS, Brazil

[3] Univ Sao Paulo, Fac Med, BR-14049 Ribeirao Preto, Brazil

[4] Univ Fed Triangulo Mineiro UFTM, Dept Clin Med, Uberaba, MG, Brazil

[5] Inst Nacl Ciencia & Tecnol Nanobiofarmaceut, BR-31270901 Belo Horizonte, MG, Brazil

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY | 2011年 / 154卷 / 03期

关键词：

Acidic phospholipase A(2); Bothrops leucurus; Cytokines; Proinflammatory profile; Snake venom; STRUCTURAL DETERMINANTS; BIOLOGICAL-ACTIVITIES; CYTOKINE RELEASE; PURIFICATION; HOMOLOG; LYS49; ASPER; MYOTOXIN; SEQUENCE; ASP-49;

D O I：

10.1016/j.cbpc.2011.06.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present study, an acidic PLA(2), designated BI-PLA(2), was isolated from Bothrops leucurus snake venom through two chromatographic steps: ion-exchange on CM-Sepharose and hydrophobic chromatography on Phenyl-Sepharose. Bl-PLA(2) was homogeneous on SDS-PAGE and when submitted to 2D electrophoresis the molecular mass was 15,000 Da and pl was 5.4. Its N-terminal sequence revealed a high homology with other Asp49 acidic PLA(2)s from snake venoms. Its specific activity was 159.9 U/mg and the indirect hemolytic activity was also higher than that of the crude venom. Bl-PLA(2) induced low myotoxic and edema activities as compared to those of the crude venom. Moreover, the enzyme was able to induce increments in IL-12p40, TNF-alpha, IL-1 beta and IL-6 levels and no variation of IL-8 and IL-10 in human PBMC stimulated in vitro, suggesting that Bl-PLA2 induces proinflammatory cytokine production by human mononuclear cells. Bothrops leucurus venom is still not extensively explored and knowledge of its components will contribute for a better understanding of its action mechanism. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：226 / 233

页数：8

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