Analysis of sister-chromatid exchanges and micronuclei in cultured human lymphocytes treated with insulin

被引：0
|
作者：
Djelic, N [1 ]
机构：
[1] Univ Belgrade, Fac Vet Med, Dept Biol, YU-11000 Belgrade, Serbia, Yugoslavia
来源：
FOLIA BIOLOGICA | 2001年 / 47卷 / 01期
关键词：
insulin; lymphocyte; sister-chromatid exchange; micronucleus; mitotic index;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Insulin is an anabolic hormone that may facilitate development of malignant diseases in various susceptible tissues due to stimulation of mitotic divisions. In this work, an evaluation of mitogenic and genotoxic effects of human recombinant insulin has been performed in cultures of human peripheral blood lymphocytes. Genotoxic effects were studied by the following test systems: (1) in vitro SCE test, and (2) cytokinesis-blocked micronucleus assay. The obtained results indicate that insulin stimulates mitotic division at an optimal concentration of 10(-8) M. On the other hand, insulin has not exhibited genotoxic properties under experimental conditions in this investigation.
引用
收藏
页码：28 / 31
页数：4
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