Bowmanella yangjiangensis sp. nov. and Amphritea pacifica sp. nov., isolated from mariculture fishponds in China

Four Gram- stain-negative, catalase-and oxidase-positive, rod-shaped and motile strains (Y26, Y57(T), ZJ14W(T) and RP18W) were isolated from mariculture fishponds in PR China. Comparisons based on 16S rRNA gene sequences showed that strains Y26 and Y57(T) share 16S rRNA gene sequence similarities in the range of 95.1-98.5 % with species of the genus Bowmanella, and strains ZJ14W(T) and RP18W share 16S rRNA gene sequence similarities in the range of 96.7 -98.8 % with species of the genus Amphritea, respectively. The genome sizes of strains Y26, Y57T, ZJ14WT and RP18W were about 4.85, 5.40, 4.70 and 4.70 Mbp with 49.5, 51.7, 51.2 and 51.3 mol% G+C content, respectively. The calculated pairwise OrthoANIu values among strains Y26, Y57(T) and species of the genus Bowmanella were in the range of 72.6-83.1 %, but the value between strains Y26 and Y57(T) was 96.2 %. The pairwise OrthoANIu values among strains ZJ14W(T), RP18W and other species of the genus Amphritea were all less than 93.9 %, but the value between strains ZJ14W(T) and RP18W was 99.3 %. Q-8 was the major respiratory quinone of strains Y26, Y57(T), ZJ14W(T) and RP18W, and the major fatty acids of these strains were all C-16 : 1 omega 7c, C-16 : 0 and C-18 : 1 omega 7c. The predominant polar lipids of strains Y26 and Y57T included phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylinositol, but strains ZJ14W(T) and RP18W only contained phosphatidylethanolamine and phosphatidylglycerol. Combining phenotypic, biochemical and genotypic characteristics, strains Y26 and Y57(T) should belong to the same species and represent a novel member of the genus Bowmanella, and strains ZJ14W(T) and RP18W should belong to the same species and represent a novel member of the genus Amphritea, for which the names Bowmanella yangjiangensis sp. nov. (type strain Y57(T)=GDMCC 1.2180(T)=KCTC 82439(T)) and Amphritea pacifica sp. nov. (type strain ZJ14W(T)=GDMCC 1.2203(T)=KCTC 82438(T)) are proposed.