Transcriptional Profiling of the Trichoderma reesei Recombinant Strain HJ48 by RNA-Seq

被引：3
|
作者：
Huang, Jun [1 ,2 ,3 ]
Wu, Renzhi [3 ]
Chen, Dong [3 ]
Wang, Qingyan [3 ]
Huang, Ribo [1 ,2 ,3 ]
机构：
[1] Guangxi Univ, State Key Lab Conservat & Utilizat Subtrop Agrobi, Nanning 530004, Guangxi, Peoples R China
[2] Guangxi Univ, Coll Life Sci & Technol, Nanning 530004, Peoples R China
[3] Guangxi Acad Sci, Natl Engn Res Ctr Nonfood Biorefinery, Nanning 530007, Guangxi, Peoples R China
来源：
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | 2016年 / 26卷 / 07期
基金：
中国国家自然科学基金;
关键词：
Trichoderma reesei; transcriptome; RNA-Seq; qPCR; DIFFERENTIAL EXPRESSION ANALYSIS; FILAMENTOUS FUNGUS; CELLULOSE; ETHANOL; IDENTIFICATION; FERMENTATION; PACKAGE;
D O I：
10.4014/jmb.1602.02003
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The ethanol production of Trichoderma reesei was improved by genome shuffling in our previous work. Using RNA-Seq, the transcriptomes of T. reesei wild-type CICC40360 and recombinant strain HJ48 were compared under fermentation conditions. Based on this analysis, we defined a set of T. reesei genes involved in ethanol production. Further expression analysis identified a series of glycolysis enzymes, which are upregulated in the recombinant strain HJ48 under fermentation conditions. The differentially expressed genes were further validated by qPCR. The present study will be helpful for future studies on ethanol fermentation as well as the roles of the involved genes. This research reveals several major differences in metabolic pathways between recombinant strain HJ48 and wild-type CICC40360, which relates to the higher ethanol production on the former, and their further research could promote the development of techniques for increasing ethanol production.
引用
收藏
页码：1242 / 1251
页数：10
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