SERCA activity is required for timely progression through G1/S

被引:19
|
作者
Simon, VR [1 ]
Moran, MF [1 ]
机构
[1] Univ Toronto, Charles H Best Inst, Banting & Best Dept Med Res, Toronto, ON M5G 1L6, Canada
关键词
D O I
10.1046/j.1365-2184.2001.00192.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Changes in intracellular Ca2+ correlate with specific events in the cell cycle. Here we investigated the role of Ca2+ in the G1 phase. HEK 293 cells were arrested in mitosis and subjected to short-term treatments that alter Ca2+ homeostasis prior to their release into G1. Treatment with thapsigargin (TG), an irreversible inhibitor of the sarco-endoplasmic reticulum Ca2+ ATPase (SERCA) lengthened the G1 phase. Moreover, TG treatment also resulted in a dramatic alteration in cellular morphology and attachment and in the reduction of MAPK activity and lower levels of cyclin D1 and cyclin E proteins. Treatments with reagents that transiently increase or decrease cytosolic Ca2+ or that temporarily inactivate SERCA did not alter any of the above parameters. Cells expressing a TG-resistant form of SERCA progressed normally through the G1/S transition after TG treatment. These results suggest that long-term SERCA inactivation affects cell cycle-dependent events and compromises progression through G1/S.
引用
收藏
页码:15 / 30
页数:16
相关论文
共 50 条
  • [31] Human cytomegalovirus mediates cell cycle progression through G1 into early S phase in terminally differentiated cells
    Sinclair, J
    Baillie, J
    Bryant, L
    Caswell, R
    JOURNAL OF GENERAL VIROLOGY, 2000, 81 : 1553 - 1565
  • [32] The FKBP12-rapamycin-binding domain is required for FKBP12-rapamycin-associated protein kinase activity and G1 progression
    Vilella-Bach, M
    Nuzzi, P
    Fang, YM
    Chen, J
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (07) : 4266 - 4272
  • [33] Gadd45g is required for timely Sry expression independently of RSPO1 activity
    Warr, Nick
    Siggers, Pam
    May, Joel
    Chalon, Nicolas
    Pope, Madeleine
    Wells, Sara
    Chaboissier, Marie-Christine
    Greenfield, Andy
    REPRODUCTION, 2022, 163 (06) : 333 - 340
  • [34] The ERK-1 function is required for HSV-1-mediated G1/S progression in HEP-2 cells and contributes to virus growth
    Colao, Ivana
    Pennisi, Rosamaria
    Venuti, Assunta
    Nygardas, Michaela
    Heikkila, Outi
    Hukkanen, Veijo
    Sciortino, Maria Teresa
    SCIENTIFIC REPORTS, 2017, 7
  • [35] The ERK-1 function is required for HSV-1-mediated G1/S progression in HEP-2 cells and contributes to virus growth
    Ivana Colao
    Rosamaria Pennisi
    Assunta Venuti
    Michaela Nygårdas
    Outi Heikkilä
    Veijo Hukkanen
    Maria Teresa Sciortino
    Scientific Reports, 7
  • [36] AML1 stimulates G1 to S progression via its transactivation domain
    Bernardin, F
    Friedman, AD
    ONCOGENE, 2002, 21 (20) : 3247 - 3252
  • [37] REGULATION OF PROGRESSION THROUGH THE G1 PHASE OF THE CELL-CYCLE BY THE RUM1(+) GENE
    MORENO, S
    NURSE, P
    NATURE, 1994, 367 (6460) : 236 - 242
  • [38] AML1 stimulates G1 to S progression via its transactivation domain
    Florence Bernardin
    Alan D Friedman
    Oncogene, 2002, 21 : 3247 - 3252
  • [39] Cactin is essential for G1 progression in Toxoplasma gondii
    Szatanek, Tomasz
    Anderson-White, Brooke R.
    Faugno-Fusci, David M.
    White, Michael
    Saeij, Jeroen P. J.
    Gubbels, Marc-Jan
    MOLECULAR MICROBIOLOGY, 2012, 84 (03) : 566 - 577
  • [40] Key effectors of signal transduction and G1 progression
    Roussel, ME
    ADVANCES IN CANCER RESEARCH, VOL 74, 1998, 74 : 1 - 24