A single amino acid substitution in the ICP27 protein of herpes simplex virus type 1 is responsible for its resistance to leptomycin B

被引：18
|
作者：
Murata, T [1 ]
Goshima, F [1 ]
Koshizuka, T [1 ]
Takakuwa, H [1 ]
Nishiyama, Y [1 ]
机构：
[1] Nagoya Univ, Sch Med, Dis Mechanism & Control Res Inst, Virol Lab,Showa Ku, Nagoya, Aichi 4668550, Japan
来源：
JOURNAL OF VIROLOGY | 2001年 / 75卷 / 02期
关键词：
D O I：
10.1128/JVI.75.2.1039-1043.2001
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Leptomycin B (LMB) is a specific inhibitor of Crm1-dependent nuclear export of proteins. The replication of herpes simplex virus (HSV) is normally highly sensitive to LMB; a resistant HSV variant, however, was isolated by serial passages of the virus. Analysis of marker transfer and viral DNA sequences revealed that a single amino acid substitution within the ICP27 gene is responsible for conferring this resistance.
引用
收藏
页码：1039 / 1043
页数：5
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