Microfluidic architectures for integrated cell lysis, lysate dialysis and cell stimulus

被引：0

作者：

Song, S ^{[1
]}

Mela, P ^{[1
]}

van den Berg, A ^{[1
]}

Kirby, BJ ^{[1
]}

机构：

[1] Sandia Natl Labs, Mircofluid Dept, Livermore, CA 94550 USA

来源：

Micro Total Analysis Systems 2004, Vol 2 | 2005年 / 297期

关键词：

cell lysis; dialysis; polymer membrane; cell stimulus;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Microfluidic architectures suitable for cell lysis, lysate dialysis, and cell stimulus are proposed. Laser-patterned nanoporous polymer membranes are fabricated in microchips using projection lithography and are used to separate cell lysate based on size and concentrate cytosolic proteins by electrophoretic concentration. Cell stimulus is achieved by rapidly changing the cell environment with aid of in-line, electrokinetic flow modulation.

引用

页码：159 / 161

页数：3

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