Application of Laser Correlation Spectroscopy for Measuring Virus Size

被引：5

作者：

Nikiforov, V. N. ^{[1
]}

Vinogradov, S. E. ^{[2
]}

Ivanov, A. V. ^{[2
]}

Efremova, E. V. ^{[3
]}

Kalnina, L. B. ^{[3
]}

Bychenko, A. B. ^{[4
]}

Tentsov, Yu. Yu. ^{[3
]}

Manykin, A. A. ^{[3
]}

机构：

[1] Moscow MV Lomonosov State Univ, Fac Phys, Moscow, Russia

[2] Russian Acad Med Sci, NN Blokhin Russian Canc Res Ctr, Moscow, Russia

[3] Minist Hlth Russian Federat, DI Ivanovsky Inst Virol, NF Gamaleya Fed Ctr Epidemiol & Microbiol, Moscow, Russia

[4] MP Chumakov Inst Poliomyelitis & Viral Encephalit, Moscow, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2016年 / 161卷 / 01期

关键词：

virus size; HIV-1; hepatitis A virus; laser correlation spectroscopy (dynamic light scattering); electron microscopy; HIV; MICROSCOPY;

D O I：

10.1007/s10517-016-3352-z

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Dynamic light scattering method or laser correlation spectroscopy was applied to evaluation of the size of viruses. We measured correlation functions of the light scattered by human immunodeficiency viruses (HIV) and hepatitis A viruses (HAV) and found that size of HIV-1 (subtype A and B) and HAV virions were 104 nm and 28 nm, respectively. Comparison of these findings with electron microscopy data for fixed samples of the same viruses showed good agreement of the results.

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页码：88 / 91

页数：4

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