Hybridization-triggered isothermal signal amplification coupled with MutS for label-free and sensitive fluorescent assay of SNPs

被引：16

作者：

Jiao, Anli ^{[1
,2
]}

Zheng, Jing ^{[1
,2
,3
,4
,5
]}

Hu, Yaping ^{[1
,2
]}

Zhu, Guizhi ^{[3
,4
,5
]}

Li, Jishan ^{[1
,2
]}

Li, Huimin ^{[1
,2
]}

Yang, Ronghua ^{[1
,2
]}

Tan, Weihong ^{[1
,2
,3
,4
,5
]}

机构：

[1] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Coll Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China

[2] Hunan Univ, Coll Mat & Engn, Changsha 410082, Hunan, Peoples R China

[3] Univ Florida, Ctr Res Bio Nano Interface, Dept Chem, Gainesville, FL 32611 USA

[4] Univ Florida, Dept Physiol & Funct Genom, Shands Canc Ctr, Gainesville, FL 32611 USA

[5] Univ Florida, UF Genet Inst, Gainesville, FL 32611 USA

来源：

CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 45期

关键词：

SINGLE-NUCLEOTIDE POLYMORPHISMS; MOLECULAR BEACONS; DNA; STRAND; ELECTROPHORESIS; OLIGONUCLEOTIDE; COMPLEMENTARY; PROBES;

D O I：

10.1039/c2cc30882g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Combining the specific recognization of MutS protein for mismatched DNA sequences with the target-driven molecular switch that acts as both the template and primer of the polymerization reaction, a new label-free and sensitive fluorescent assay strategy for specific single-stranded DNA sequences or SNPs is proposed.

引用

页码：5659 / 5661

页数：3

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