4D confocal microscopy method for drug localization in the skin

被引：0

作者：

Maeder, Ulf ^{[1
]}

Bergmann, Thorsten ^{[1
]}

Burg, Jan Michael ^{[1
]}

Beer, Sebastian ^{[1
]}

Schlupp, Peggy ^{[2
]}

Schmidts, Thomas ^{[2
]}

Heverhagen, Johannes T. ^{[3
]}

Runkel, Frank ^{[2
]}

Fiebich, Martin ^{[1
]}

机构：

[1] Univ Appl Sci, Tech Hsch Mittelhessen, Inst Med Phys & Radiat Protect, Friedberg, Germany

[2] Univ Appl Sci, Tech Hsch Mittelhessen, Inst Biopharmacetical Tech, Friedberg, Germany

[3] Univ Marburg, Dept Diagnostic Radio, Marburg, Germany

来源：

ADVANCED MICROSCOPY TECHNIQUES II | 2011年 / 8086卷

关键词：

transdermal drug transport; skin permeation study; confocal microscopy; spectral unmixing; drug permeation depth; local drug distribution; DELIVERY;

D O I：

10.1117/12.889497

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

A 4D confocal microscopy (xyz lambda) method for measuring the drug distribution in skin samples after a permeation study is investigated. This approach can be applied to compare different drug carrier systems in pharmaceutical research studies. For the development of this detection scheme phantom permeation studies and preliminary skin measurements are carried out. The phantom studies are used to detect the permeation depth and the localization of the external applied fluorescent dye naphthofluorescein that is used as a model agent. The skin study shows the feasibility of the method for real tissue. For the differentiation of tissue/phantom and the dye, spectral unmixing is performed using the spectral information detected by a confocal microscope. The results show that it is possible to identify and localize external dyes in the phantoms as well as in the skin samples.

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页数：6

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