DNA barcoding in medicinal plants: Testing the potential of a proposed barcoding marker for identification of Uncaria species from China

被引：17

作者：

Zhang, Zhong-Lian ^{[1
]}

Song, Mei-Fang ^{[1
]}

Guan, Yan-Hong ^{[1
]}

Li, Hai-Tao ^{[1
]}

Niu, Ying-Feng ^{[1
]}

Zhang, Li-Xia ^{[1
]}

Ma, Xiao-Jun ^{[1
,2
]}

机构：

[1] Chinese Acad Med Sci, Peking Union Med Coll, Yunnan Branch,Inst Med Plant Dev, Key Lab Dai & Southern Med Xishuangbanna Dai Auto, Jinghong 666100, Peoples R China

[2] Chinese Acad Med Sci, Peking Union Med Coll, Inst Med Plant Dev, Beijing 100193, Peoples R China

来源：

BIOCHEMICAL SYSTEMATICS AND ECOLOGY | 2015年 / 60卷

关键词：

Uncaria; DNA barcoding; Internal transcribed spacer-2 (ITS2); LAND PLANTS; RBCL;

D O I：

10.1016/j.bse.2015.02.017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA barcoding, an increasingly popular mean of species identification, has been widely used for global species identification despite a consensus not being reached regarding which DNA sequences can be used as the best plant barcodes. In this study, we tested the feasibility of five candidate DNA barcodes (nrITS, nrITS2, matk, rbcL and trnH-psbA) for identifying Uncaria species. We collected a total of 54 specimens of 10 Uncaria species across its distributional range. BLAST, barcoding gaps, tree-based methods and TAXONDNA analysis were used to investigate the molecular identification capability of the candidate DNA barcodes. The results showed that the ITS2 is most suitable as a candidate DNA barcode for identification of medicinal plants of the genus Uncaria. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：8 / 14

页数：7

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