Mechanisms of acupuncture and moxibustion in regulation of epithelial cell apoptosis in rat ulcerative colitis

被引：27
|
作者：
Wu, Huan-Gan [1 ,2 ]
Gong, Xiao [1 ,2 ]
Yao, Li-Qing [3 ]
Zhang, Wei [1 ,2 ]
Shi, Yin [1 ,2 ]
Liu, Hui-Rong [1 ,2 ]
Gong, Ye-Jing [1 ,2 ]
Zhou, Li-Bin [4 ]
Zhu, Yi [1 ,2 ]
机构：
[1] Shanghai Inst Acupuncture Moxibust & Meridians, Shanghai 200030, Peoples R China
[2] Shanghai Reaserch Ctr Acupuncture & Meridian, Shanghai 201200, Peoples R China
[3] Fudan Univ, Zhongshan Hosp, Shanghai 200032, Peoples R China
[4] Shanghai Inst Endocrinol, RuiJin Hosp, Shanghai 200025, Peoples R China
来源：
WORLD JOURNAL OF GASTROENTEROLOGY | 2004年 / 10卷 / 05期
基金：
中国国家自然科学基金;
关键词：
D O I：
10.3748/wjg.v10.i5.682
中图分类号：
R57 [消化系及腹部疾病];
学科分类号：
摘要：
AIM: To investigate the effect of acupuncture and moxibustion on epithelial cell apoptosis and expression of Bcl-2, Bax, fas and FasL proteins in rat ulcerative colitis. METHODS: A rat model of ulcerative colitis was estabelished by immunological methods and local stimulation. All rats were randomly divided into model control group (MC), electro-acupuncture group (EA), herbs-partition moxibustion group (HPM). Normal rats were used as normal control group (NC). Epithelial cell apoptosis and expression of Bcl-2, Bax, fas and FasL proteins were detected by TUNEL and immunohistochemiscal method respectively. RESULTS: The number of epithelial cell apoptosis in MC was significantly higher than that in NC, and was markedly decreased after the treatment with herbs-partition moxibustion or electro-acupuncture. The expression of Bcl-2, Bax, fas and FasL in colonic epithelial cells in MC was higher than that in NC, and was markedly down-regulated by herbs-partition moxibustion or electro-acupuncture treatment. CONCLUSION: The pathogenesis of ulcerative colitis in rats involves abnormality of apoptosis. Acupuncture and moxibustion can regulate the expression of Bcl-2, Bax, fas and FasL proteins and inhibit the apoptosis of epithelial cells of ulcerative colitis in rats by Bcl-2/Bax, fas/FasL pathways.
引用
收藏
页码：682 / 688
页数：7
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