Direct measurement of oxygen concentration inside cultured cartilage for relating to spatial growth of rabbit chondrocytes

被引：10
|
作者：
Kino-oka, Masahiro [2 ]
Kagita, Shogo [1 ]
Nadzir, Masrina Mohd [1 ]
Inoue, Hirofumi [1 ]
Sugawara, Katsura [3 ]
Taya, Masahito [1 ]
机构：
[1] Osaka Univ, Div Chem Engn, Grad Sch Engn Sci, Osaka 5608531, Japan
[2] Osaka Univ, Dept Biotechnol, Grad Sch Engn, Suita, Osaka 5650871, Japan
[3] Japan Tissue Engn Co Ltd, Aichi 4430022, Japan
来源：
JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2010年 / 110卷 / 03期
关键词：
Tissue engineering; Cultured cartilage; Chondrocytes; Dissolved oxygen measurement; Spatial cell distribution; HUMAN ARTICULAR-CARTILAGE; ENGINEERED CARTILAGE; CELL-DENSITY; IN-VITRO; TISSUE; GRADIENTS; VIABILITY; TENSION; GEL;
D O I：
10.1016/j.jbiosc.2010.03.009
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
In static culture of rabbit chondrocytes in collagen gel, the direct measurement of dissolved oxygen (DO) concentration revealed that the DO level at the top surface of gel decreased due to an increase in overall cell density with elapsed time. The local cell density at the top surface on day 21 was 5.7 x 10(7) cells/cm(3), being 11 times that at the bottom of gel. This heterogeneity of cell distribution in the gel was considered to occur by limitation of oxygen supply into a deeper part of the gel. In shaking culture using a dish with gas-permeable film, the DO level was enhanced inside the gel and the overall cell density in the gel was achieved to be 2.9 times that in the static culture. (C) 2010, The Society for Biotechnology, Japan. All rights reserved.
引用
收藏
页码：363 / 366
页数：4
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