Facile enzymatic synthesis of sugar 1-phosphates as substrates for phosphorylases using anomeric kinases

被引：21
|
作者：
Liu, Yuan [1 ]
Nishimoto, Mamoru [1 ]
Kitaoka, Motomitsu [1 ]
机构：
[1] Natl Agr & Food Res Org, Natl Food Res Inst, Tsukuba, Ibaraki 3058642, Japan
来源：
CARBOHYDRATE RESEARCH | 2015年 / 401卷
关键词：
Sugar; 1-phosphate; Anomeric kinase; Pyruvate kinase; Electrodialysis; Substrate for phosphorylase; GLYCOSIDE PHOSPHORYLASES; GLUCOSE; 1-PHOSPHATE; ENZYMES; PATHWAY; IDENTIFICATION; PURIFICATION; METABOLISM; DISCOVERY; BACTERIUM; EXCHANGE;
D O I：
10.1016/j.carres.2014.10.014
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Three sugar 1-phosphates that are donor substrates for phosphorylases were produced at the gram scale from phosphoenolpyruvic acid and the corresponding sugars by the combined action of pyruvate kinase and the corresponding anomeric kinases in good yields. These sugar 1-phosphates were purified through two electrodialysis steps. alpha-D-Galactose 1-phosphate was finally isolated as crystals of dipotassium salts. alpha-D-Mannose 1-phosphate and 2-acetamido-2-deoxy-alpha-D-glucose 1-phosphate were isolated as crystals of bis(cyclohexylammonium) salts. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1 / 4
页数：4
相关论文
共 50 条
  • [41] Enzymatic peptide synthesis using inverse substrates: Biocatalysts investigation in industrial waste
    Sekizaki, Haruo
    Itoh, Kunihiko
    Toyota, Eiko
    Horita, Kiyoshi
    Noguchi, Yukari
    Tanizawa, Kazutaka
    Peptides 2004, Proceedings: BRIDGES BETWEEN DISCIPLINES, 2005, : 271 - 272
  • [42] ENZYMATIC PEPTIDE SYNTHESIS USING INVERSE SUBSTRATES: BIOCATALYSTS INVESTIGATIN IN INDUSTRIAL WASTE
    Sekizaki, H.
    Itoh, K.
    Toyota, E.
    Horita, K.
    Noguchi, Y.
    Tanizawa, K.
    JOURNAL OF PEPTIDE SCIENCE, 2004, 10 : 135 - 135
  • [43] A FACILE ENZYMATIC-SYNTHESIS OF SPHINGOSINE-1-PHOSPHATE AND DIHYDROSPHINGOSINE-1-PHOSPHATE
    VANVELDHOVEN, PP
    FOGLESONG, RJ
    BELL, RM
    JOURNAL OF LIPID RESEARCH, 1989, 30 (04) : 611 - 616
  • [44] One-step, stereocontrolled synthesis of glycosyl 1-phosphates, uridine-5′-diphosphogalactose, and uridine-5′-diphosphoglucose from unprotected glycosyl donors
    Hanessian, S
    Lu, PP
    Ishida, H
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (51) : 13296 - 13300
  • [45] A novel synthesis of D-galactofuranosyl, D-glucofuranosyl and D-mannofuranosyl 1-phosphates based on remote activation of new and free hexofuranosyl donors
    Ferrières, V
    Blanchard, S
    Fischer, D
    Plusquellec, D
    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2002, 12 (24) : 3515 - 3518
  • [46] A FACILE ENZYMATIC-SYNTHESIS OF CELLOOLIGOSACCHARIDE DERIVATIVES USING BETA-LACTOSYL FLUORIDE
    SHODA, S
    KAWASAKI, T
    OBATA, K
    KOBAYASHI, S
    CARBOHYDRATE RESEARCH, 1993, 249 (01) : 127 - 137
  • [47] Enzymatic synthesis of 1-deoxysugar-phosphates using E-coli 1-deoxy-D-xylulose 5-phosphate synthase
    Querol, J
    Grosdemange-Billiard, C
    Rohmer, M
    Boronat, A
    Imperial, S
    TETRAHEDRON LETTERS, 2002, 43 (46) : 8265 - 8268
  • [48] Facile Synthesis of Novel Glycosyl Carboxamide with Sugar in Furanose and Pyranose form Using Benzotriazole Methodology
    Kale, Raju R.
    Prasad, Virendra
    Tiwari, Vinod K.
    LETTERS IN ORGANIC CHEMISTRY, 2010, 7 (02) : 136 - 143
  • [49] Facile synthesis of tough metallosupramolecular hydrogels by using phosphates as temporary ligands of ferric ions to avoid inhibition of polymerization
    Fang, Lingtao
    Hu, Jian
    Zhang, Chuan Wei
    Wei, Jialun
    Yu, Hai Chao
    Zheng, Si Yu
    Wu, Zi Liang
    Zheng, Qiang
    JOURNAL OF POLYMER SCIENCE, 2022, 60 (15) : 2280 - 2288
  • [50] A novel method for synthesis of chitobiose via enzymatic glycosylation using a sugar oxazoline as glycosyl donor
    Kobayashi, S
    Kiyosada, T
    Shoda, S
    TETRAHEDRON LETTERS, 1997, 38 (12) : 2111 - 2112
12345