Facile enzymatic synthesis of sugar 1-phosphates as substrates for phosphorylases using anomeric kinases

被引：21

作者：

Liu, Yuan ^{[1
]}

Nishimoto, Mamoru ^{[1
]}

Kitaoka, Motomitsu ^{[1
]}

机构：

[1] Natl Agr & Food Res Org, Natl Food Res Inst, Tsukuba, Ibaraki 3058642, Japan

来源：

CARBOHYDRATE RESEARCH | 2015年 / 401卷

关键词：

Sugar; 1-phosphate; Anomeric kinase; Pyruvate kinase; Electrodialysis; Substrate for phosphorylase; GLYCOSIDE PHOSPHORYLASES; GLUCOSE; 1-PHOSPHATE; ENZYMES; PATHWAY; IDENTIFICATION; PURIFICATION; METABOLISM; DISCOVERY; BACTERIUM; EXCHANGE;

D O I：

10.1016/j.carres.2014.10.014

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three sugar 1-phosphates that are donor substrates for phosphorylases were produced at the gram scale from phosphoenolpyruvic acid and the corresponding sugars by the combined action of pyruvate kinase and the corresponding anomeric kinases in good yields. These sugar 1-phosphates were purified through two electrodialysis steps. alpha-D-Galactose 1-phosphate was finally isolated as crystals of dipotassium salts. alpha-D-Mannose 1-phosphate and 2-acetamido-2-deoxy-alpha-D-glucose 1-phosphate were isolated as crystals of bis(cyclohexylammonium) salts. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：1 / 4

页数：4

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