PulseNet standardized protocol for subtyping Listeria monocytogenes by macrorestriction and pulsed-field gel electrophoresis

被引：454
|
作者：
Graves, LM [1 ]
Swaminathan, B [1 ]
机构：
[1] Ctr Dis Control & Prevent, Foodbourne & Diarrheal Dis Branch, Div Bacterial & Mycot Dis, Natl Ctr Infect Dis, Atlanta, GA 30333 USA
来源：
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 2001年 / 65卷 / 1-2期
关键词：
PulseNet; Listeria monocytogenes; pulsed-field gel electrophoresis; subtyping; epidemiology;
D O I：
10.1016/S0168-1605(00)00501-8
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
PulseNet is a national network of pubic health and food regulatory laboratories established in the US to detect clusters of foodborne disease and respond quickly to foodborne outbreak investigations. PulseNet laboratories: currently subtype Escherichia coli O157:H7, non-typhoidal Salmonella. and Shigella isolates by a highly standardized I-day pulsed-field gel electrophoresis (PFGE). and exchange normalized DNA "fingerprint" patterns via the Internet. We describe a standardized molecular subtyping protocol for subtyping Listeria monocytogenes that was recently added to PulseNet. The subtyping can be completed within 30 h from the time a pure culture of the bacteria is obtained. (C) 2001 Elsevier science B.V. All rights reserved.
引用
收藏
页码：55 / 62
页数：8
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