Monoclonal antibody against mouse CAR following genetic immunization

被引：4
|
作者：
Carson, SD [1 ]
Switzer, BL [1 ]
Tracy, SM [1 ]
Chapman, NM [1 ]
机构：
[1] Univ Nebraska, Med Ctr, Dept Pathol & Microbiol, Omaha, NE 68198 USA
来源：
HYBRIDOMA AND HYBRIDOMICS | 2004年 / 23卷 / 01期
关键词：
D O I：
10.1089/153685904322771980
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
To broaden our repertoire of monoclonal antibodies against CAR ( coxsackievirus and adenovirus receptor), we inoculated mice with an expression vector containing the cDNA encoding human CAR extracellular and transmembrane sequence, and boosted the response by inoculation with soluble human CAR protein produced in E. coli. Of the hybridomas obtained following this immunization protocol, one secreted IgG with exceptional reactivity against mouse CAR. Since CAR has been shown to form dimers, expression of human CAR in cells that express mouse CAR may have stimulated the host immune system to recognize endogenous CAR in heterodimers.
引用
收藏
页码：19 / 22
页数：4
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