Novel preparation of organic polymer-based monolithic column by microwave irradiation

Microwave irradiation was firstly attempted for the preparation of organic-based monoliths of poly(styrene-divinylbenzene-methacrylic acid), which single step in situ polymerization was carried out during 15 min. The column permeability, electrophoretic and chromatographic behaviors were comparatively evaluated using pressure-assisted CEC, CEC and low pressure-driven separation modes. The largest theoretical plates for the preparing column could be close to 18,0000 plates/m for thiourea in the mode of p-CEC. It provided a viable alternative to traditional initiation means for the preparation of monolithic capillary columns. (c) 2007 Yu Ping Zhang. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.