Postnatal development of cell types in the hamster Harderian gland

The morphology, frequency, and distribution of mitotic cells in the epithelium of the Harderian gland was studied in the male and female hamster from birth to the ninetieth day of postnatal life using light and electron microscopic techniques. The results obtained show that there is a gradual decline in the mitotic activity of the Harderian gland as the animals become older which continues until sexual maturity is reached. The study did not find any morphological evidence for the existence of separate precursor cells for the different secretory cell types of the gland. Secretory cells seem to be a homogeneous population in the glands of hamsters younger than 20 days. Cells that could be interpreted as undifferentiated stem cells were not observed. Mitotic cells were observed randomly distributed within tubulo-alveoli, similar to those in interphase. The first sex differences were observed at day 20, when type II cells appeared in male glands. From this point, the percentage of type II cells rapidly increased in male glands. This increase was not accompanied by an increase in the global mitotic activity. However, at this age, male type II cells develop an intense mitotic activity. The observations obtained are in accordance with the hypothesis that the type I and type II cells have the same cell precursor. (C) 1996 Wiley-Liss, Inc.