Repetitive-sequence-based PCR using the DiversiLab system for identification of aspergillus species

被引：18

作者：

Hansen, Dewey ^{[1
]}

Healy, Mimi ^{[2
]}

Reece, Kristy ^{[2
]}

Smith, Cheryl ^{[2
]}

Woods, Gail L. ^{[1
,3
,4
]}

机构：

[1] Associated Reg & Univ Pathologists Inc, Infect Dis Lab, Salt Lake City, UT USA

[2] Bacterial Barcodes Inc, Athens, GA USA

[3] Associated Reg & Univ Pathologists Inc, Inst Clin & Expt Pathol, Salt Lake City, UT USA

[4] Univ Utah, Sch Med, Dept Pathol, Salt Lake City, UT USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2008年 / 46卷 / 05期

关键词：

D O I：

10.1128/JCM.00956-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Repetitive-sequence-based PCR (rep-PCR) using the DiversiLab system was investigated for identification of Aspergillus. Ninety-five clinical isolates, identified by conventional methods, and five ATCC strains were tested. Sequencing of the internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2) was performed on 2 isolates with discrepant rep-PCR and conventional results and 15 randomly selected outlier isolates. One isolate not identified by sequencing was excluded from analysis. After resolving discrepancies, all but one A. glaucus strain had >= 85% similarity to one or more strains of the same Aspergillus species in the mold database, thereby providing accurate identification. No isolate was misidentified.

引用

页码：1835 / 1839

页数：5

共 50 条

[41] PCR identification system for the genus Aspergillus and three major pathogenic species:: Aspergillus fumigatus, Aspergillus flavus and Aspergillus niger
Sugita, C
Makimura, K
Uchida, K
Yamaguchi, H
Nagai, A
[J]. MEDICAL MYCOLOGY, 2004, 42 (05) : 433 - 437
[42] Comparison of a Commercially Available Repetitive-Element PCR System (DiversiLab) with PCR Ribotyping for Typing of Clostridium difficile Strains
Eckert, C.
Van Broeck, J.
Spigaglia, P.
Burghoffer, B.
Delmee, M.
Mastrantonio, P.
Barbut, F.
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2011, 49 (09) : 3352 - 3354
[43] Clinical evaluation of repetitive sequence-based polymerase chain reaction using the DiversiLab System for strain typing of vancomycin-resistant enterococci
Pounder, JI
Shutt, CK
Schaecher, BJ
Woods, GL
[J]. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2006, 54 (03) : 183 - 187
[44] Repetitive-Sequence-Based Genotyping and Clonal Relationship Analysis of Acinetobacter baumannii in Nosocomial Infections
Jiang, Wei
Liu, Hua
Yang, Yong-Chang
Xiao, Dai-Wen
Yu, Hua
Huang, Wen-Fang
Zhou, Zhong-Hua
Chen, Shu-Lan
[J]. LABMEDICINE, 2012, 43 (03): : 86 - 90
[45] Comparison of an Automated Repetitive-Sequence-Based PCR Microbial Typing System with Pulsed-Field Gel Electrophoresis for Molecular Typing of Vancomycin-Resistant Enterococcus faecium
Chuang, Yu-Chung
Wang, Jann-Tay
Chen, Mei-Ling
Chen, Yee-Chun
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (08) : 2897 - 2901
[46] Genomic fingerprints of Salmonella species generated with repetitive element sequence-based PCR
Koh-Luar S.I.
Choo G.H.B.
Tan S.L.R.
Tan C.W.K.
[J]. World Journal of Microbiology and Biotechnology, 1997, 14 (1) : 17 - 22
[47] Repetitive element sequence-based PCR for species and strain discrimination in the genus Listeria
Jersek, B
Tcherneva, E
Rijpens, N
Herman, L
[J]. LETTERS IN APPLIED MICROBIOLOGY, 1996, 23 (01) : 55 - 60
[48] Genomic fingerprints of Salmonella species generated with repetitive element sequence-based PCR
Koh-Luar, SI
Choo, GHB
Tan, SLR
Tan, CWK
[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 1998, 14 (01): : 17 - 22
[49] Identification of Burkholderia andropogonis with a Repetitive Sequence BOX Element and PCR
Bruce A. Ramundo
Larry E. Claflin
[J]. Current Microbiology, 2005, 50 : 52 - 56
[50] Identification of Burkholderia andropogonis with a repetitive sequence BOX element and PCR
Ramundo, BA
Claflin, LE
[J]. CURRENT MICROBIOLOGY, 2005, 50 (01) : 52 - 56

← 1 2 3 4 5 →