Repetitive-sequence-based PCR using the DiversiLab system for identification of aspergillus species

被引：18
|
作者：
Hansen, Dewey [1 ]
Healy, Mimi [2 ]
Reece, Kristy [2 ]
Smith, Cheryl [2 ]
Woods, Gail L. [1 ,3 ,4 ]
机构：
[1] Associated Reg & Univ Pathologists Inc, Infect Dis Lab, Salt Lake City, UT USA
[2] Bacterial Barcodes Inc, Athens, GA USA
[3] Associated Reg & Univ Pathologists Inc, Inst Clin & Expt Pathol, Salt Lake City, UT USA
[4] Univ Utah, Sch Med, Dept Pathol, Salt Lake City, UT USA
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2008年 / 46卷 / 05期
关键词：
D O I：
10.1128/JCM.00956-07
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Repetitive-sequence-based PCR (rep-PCR) using the DiversiLab system was investigated for identification of Aspergillus. Ninety-five clinical isolates, identified by conventional methods, and five ATCC strains were tested. Sequencing of the internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2) was performed on 2 isolates with discrepant rep-PCR and conventional results and 15 randomly selected outlier isolates. One isolate not identified by sequencing was excluded from analysis. After resolving discrepancies, all but one A. glaucus strain had >= 85% similarity to one or more strains of the same Aspergillus species in the mold database, thereby providing accurate identification. No isolate was misidentified.
引用
收藏
页码：1835 / 1839
页数：5
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