In vitro evaluation of the probiotic properties of human intestinal Bifidobacterium species and selection of new probiotic candidates

被引:36
|
作者
Delgado, S. [1 ,2 ]
O'Sullivan, E. [2 ]
Fitzgerald, G. [2 ]
Mayo, B. [1 ]
机构
[1] Inst Prod Lacteos Asturias, Dept Microbiol & Bioquim, Villaviciosa 33300, Asturias, Spain
[2] Natl Univ Ireland Univ Coll Cork, Natl Food Biotechnol Ctr, Dept Microbiol, Cork, Ireland
关键词
bifidobacteria; Bifidobacterium; human gastrointestinal tract; intestinal microbiology; probiotics;
D O I
10.1111/j.1365-2672.2007.03642.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Aims: The aim of this work was to identify and select new probiotic strains among majority intestinal bifidobacterial species from healthy Spaniards. Methods and Results: One hundred and eighty isolates belonging to seven Bifidobacterium species were subjected to a subtractive system of in vitro analysis addressing beneficial and undesirable traits. Approx. 45% of the isolates were able to grow in 2% bovine bile, and about 20% of these grew at pH 3.5. Undesirable enzymatic activities, such as alpha-chymotrypsin, beta-glucuronidase and N-acetyl-beta-glucosaminidase were not detected. Atypical antibiotic resistances were not observed, except for tetracycline resistance in a single strain. Intestinal pathogens were inhibited to some extent by all analysed strains. All selected strains adhered to human epithelial cells in a strain-dependent manner, and none was able to degrade pig mucin. Conclusions: Based on these in vitro analyses, strains of Bifidobacterium catenulatum, Bifidobacterium longum and Bifidobacterium pseudocatenulatum are here proposed as new probiotic candidates. Significance and Impact of the Study: Although in vivo analyses are still needed, these strains belonging to unusual species in the portfolio of probiotic suppliers are thought to be more appropriated than those currently in use, as they show desirable properties and are preponderant among human intestinal populations.
引用
收藏
页码:1119 / 1127
页数:9
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