Suspended-drop electroporation for high-throughput delivery of biomolecules into cells

被引：30

作者：

Guignet, Emmanuel G. ^{[1
]}

Meyer, Tobias ^{[1
]}

机构：

[1] Stanford Univ, Sch Med, Dept Chem & Syst Biol, Bio X Program,Clark Ctr, Stanford, CA 94305 USA

来源：

NATURE METHODS | 2008年 / 5卷 / 05期

关键词：

D O I：

10.1038/NMETH.1201

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a high-throughput method that enables efficient delivery of biomolecules into cells. The device consists of an array of 96 suspended electrode pairs, where small sample volumes are top-loaded, electroporated and bottom-ejected into 96-well plates. We demonstrate the use of this suspended-drop electroporation (SDE) device to effectively introduce fluorescent dextran, small interfering RNA (siRNA) or cDNA into primary neurons, differentiated neutrophils and other cell types with conventionally low transfection rates.

引用

页码：393 / 395

页数：3

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