Site-directed spin labeling of proteins for distance measurements in vitro and in cells

被引：74
|
作者：
Roser, P. [1 ,2 ]
Schmidt, M. J. [1 ,2 ]
Drescher, M. [1 ,2 ]
Summerer, D. [3 ]
机构：
[1] Univ Konstanz, Dept Chem, Zukunftskolleg, Univ Str 10, D-78457 Constance, Germany
[2] Univ Konstanz, Konstanz Res Sch Chem Biol, Univ Str 10, D-78457 Constance, Germany
[3] Tech Univ Dortmund, Dept Chem & Chem Biol, Otto Hahn Str 6, D-44227 Dortmund, Germany
来源：
ORGANIC & BIOMOLECULAR CHEMISTRY | 2016年 / 14卷 / 24期
关键词：
ELECTRON-ELECTRON RESONANCE; ESCHERICHIA-COLI-CELLS; UNNATURAL AMINO-ACID; EPR SPECTROSCOPY; SIDE-CHAINS; PULSED EPR; RELAXATION-ENHANCEMENT; CONFORMATIONAL-CHANGES; MEMBRANE TRANSPORTER; TRITYL RADICALS;
D O I：
10.1039/c6ob00473c
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
Site-directed spin labeling (SDSL) in combination with electron paramagnetic resonance (EPR) spectroscopy allows studying the structure, dynamics, and interactions of proteins via distance measurements in the nanometer range. We here give an overview of available spin labels, the strategies for their introduction into proteins, and the associated potentials for protein structural studies in vitro and in the context of living cells.
引用
收藏
页码：5468 / 5476
页数：9
相关论文
共 50 条
  • [31] Attaching a spin to a protein - site-directed spin labeling in structural biology
    Czogalla, Aleksander
    Pieciul, Aldona
    Jezierski, Adam
    Sikorski, Aleksander F.
    ACTA BIOCHIMICA POLONICA, 2007, 54 (02) : 235 - 244
  • [32] Site-directed spin-labeling of myosin II mutants
    Klein, JC
    Blakely, SE
    Surek, JT
    Titus, MA
    Thomas, DD
    BIOPHYSICAL JOURNAL, 2004, 86 (01) : 484A - 484A
  • [33] Expanding the Genetic Code for Site-Directed Spin-Labeling
    Braun, Theresa
    Drescher, Malte
    Summerer, Daniel
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2019, 20 (02)
  • [34] SITE-DIRECTED SPIN-LABELING STUDIES OF THE BACTERIORHODOPSIN PHOTOCYCLE
    STEINHOFF, HJ
    MOLLAAGHABABA, R
    KHORANA, G
    HUBBELL, WL
    BIOPHYSICAL JOURNAL, 1994, 66 (02) : A40 - A40
  • [35] Site-directed spin labeling to detect actomyosin structural transitions
    Thomas, DD
    Titus, MA
    Blakely, S
    Surek, JT
    Klein, JC
    Korman, V
    Prochniewicz, E
    Nesmelov, Y
    BIOPHYSICAL JOURNAL, 2004, 86 (01) : 515A - 515A
  • [36] Methods and applications of site-directed spin Labeling EPR Spectroscopy
    Klug, Candice S.
    Feix, Jimmy B.
    BIOPHYSICAL TOOLS FOR BIOLOGISTS: VOL 1 IN VITRO TECHNIQUES, 2008, 84 : 617 - 658
  • [37] Site-directed spin labeling EPR spectroscopy in protein research
    Klare, Johann P.
    BIOLOGICAL CHEMISTRY, 2013, 394 (10) : 1281 - 1300
  • [38] Investigation of α-synuclein fibril structure by site-directed spin labeling
    Chen, Min
    Margittai, Martin
    Chen, Jeannie
    Langen, Ralf
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (34) : 24970 - 24979
  • [39] Orthogonal Tyrosine and Cysteine Site-Directed Spin Labeling for Dipolar Pulse EPR Spectroscopy on Proteins
    Gmeiner, Christoph
    Klose, Daniel
    Mileo, Elisabetta
    Belle, Valerie
    Marque, Sylvain R. A.
    Dorn, Georg
    Allain, Frederic H. T.
    Guigliarelli, Bruno
    Jeschke, Gunnar
    Yulikov, Maxim
    JOURNAL OF PHYSICAL CHEMISTRY LETTERS, 2017, 8 (19): : 4852 - 4857
  • [40] New limits of sensitivity of site-directed spin labeling electron paramagnetic resonance for membrane proteins
    Bordignon, Enrica
    Bleicken, Stephanie
    BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2018, 1860 (04): : 841 - 853
12345