Site-directed spin labeling of proteins for distance measurements in vitro and in cells

被引：74
|
作者：
Roser, P. [1 ,2 ]
Schmidt, M. J. [1 ,2 ]
Drescher, M. [1 ,2 ]
Summerer, D. [3 ]
机构：
[1] Univ Konstanz, Dept Chem, Zukunftskolleg, Univ Str 10, D-78457 Constance, Germany
[2] Univ Konstanz, Konstanz Res Sch Chem Biol, Univ Str 10, D-78457 Constance, Germany
[3] Tech Univ Dortmund, Dept Chem & Chem Biol, Otto Hahn Str 6, D-44227 Dortmund, Germany
来源：
ORGANIC & BIOMOLECULAR CHEMISTRY | 2016年 / 14卷 / 24期
关键词：
ELECTRON-ELECTRON RESONANCE; ESCHERICHIA-COLI-CELLS; UNNATURAL AMINO-ACID; EPR SPECTROSCOPY; SIDE-CHAINS; PULSED EPR; RELAXATION-ENHANCEMENT; CONFORMATIONAL-CHANGES; MEMBRANE TRANSPORTER; TRITYL RADICALS;
D O I：
10.1039/c6ob00473c
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
Site-directed spin labeling (SDSL) in combination with electron paramagnetic resonance (EPR) spectroscopy allows studying the structure, dynamics, and interactions of proteins via distance measurements in the nanometer range. We here give an overview of available spin labels, the strategies for their introduction into proteins, and the associated potentials for protein structural studies in vitro and in the context of living cells.
引用
收藏
页码：5468 / 5476
页数：9
相关论文
共 50 条
  • [1] Nanometer distance measurements in RNA using site-directed spin Labeling
    Cai, Qi
    Kusnetzow, Ana Karin
    Hideg, Kalman
    Price, Eric A.
    Haworth, Ian S.
    Qin, Peter Z.
    BIOPHYSICAL JOURNAL, 2007, 93 (06) : 2110 - 2117
  • [2] Site-Directed Spin Labeling of Membrane Proteins
    Bordignon, Enrica
    EPR SPECTROSCOPY: APPLICATIONS IN CHEMISTRY AND BIOLOGY, 2012, 321 : 121 - 157
  • [3] Technological advances in site-directed spin labeling of proteins
    Hubbell, Wayne L.
    Lopez, Carlos J.
    Altenbach, Christian
    Yang, Zhongyu
    CURRENT OPINION IN STRUCTURAL BIOLOGY, 2013, 23 (05) : 725 - 733
  • [4] Recent advances in site-directed spin labeling of proteins
    Hubbell, WL
    Gross, A
    Langen, R
    Lietzow, MA
    CURRENT OPINION IN STRUCTURAL BIOLOGY, 1998, 8 (05) : 649 - 656
  • [5] Watching proteins move using site-directed spin labeling
    Hubbell, WL
    Mchaourab, HS
    Altenbach, C
    Lietzow, MA
    STRUCTURE, 1996, 4 (07) : 779 - 783
  • [6] Site-directed spin labeling of membrane proteins by peptide synthesis
    Karim, CB
    Kirby, TL
    Thomas, DD
    BIOPHYSICAL JOURNAL, 2004, 86 (01) : 35A - 35A
  • [7] Site-Directed Spin Labeling EPR for Studying Membrane Proteins
    Sahu, Indra D.
    Lorigan, Gary A.
    BIOMED RESEARCH INTERNATIONAL, 2018, 2018
  • [8] Introduction and future of site-directed spin labeling of membrane proteins
    Hemminga, Marcus A.
    ESR SPECTROSCOPY IN MEMBRANE BIOPHYSICS, 2007, 27 : 1 - 16
  • [9] A distance ruler for RNA using EPR and site-directed spin labeling
    Kim, NK
    Murali, A
    DeRose, VJ
    CHEMISTRY & BIOLOGY, 2004, 11 (07): : 939 - 948
  • [10] Motional restrictions of membrane proteins: A site-directed spin labeling study
    Stopar, David
    Strancar, Janez
    Spruijt, Ruud B.
    Hemminga, Marcus A.
    BIOPHYSICAL JOURNAL, 2006, 91 (09) : 3341 - 3348
12345