Heritability of genetic variants of resistin gene in patients with coronary artery disease: A family-based study

Objective: The resistin gene (RETN) -420 C>G and +299 G>A polymorphism was investigated in a case-control study from forty complex Pakistani families with coronary artery disease (CAD) history. Heritability of the susceptible/variant alleles was investigated from parent-offspring trios in these families. Method: Resistin levels were determined from 239 individuals by enzyme-linked immunosorbent assay. Genotyping was performed using polymerase chain reaction and restriction fragment length polymorphism. Results: Elevated resistin levels were observed from CAD cases vs. controls (p<0.0001). The REIN -420 C>G and +299 G>A polymorphism was more prevalent in cases vs. controls (p<0.0001). The transmission disequilibrium test revealed a significant association of the -420 and +299 polymorphism with CAD (chi(2)=34.4, p<0.0001 and chi(2)=31.6, p<0.0001, respectively). Conclusion: Elevated resistin, and the RETN -420 C>G and +299 G>A polymorphism may contribute to familial CAD. The 420 and +299 variant alleles are transmitted more frequently from parent to affected offspring. This is the first report on the association of the REIN +299 G>A polymorphism with CAD. (C) 2011 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.