Cloning and Sequencing of Inulinase and β-Fructofuranosidase Genes of a Deep-Sea Microbulbifer Species and Properties of Recombinant Enzymes

被引：17
|
作者：
Kobayashi, Tohru [1 ]
Uchimura, Kohsuke [1 ]
Deguchi, Shigeru [1 ]
Horikoshi, Koki [1 ]
机构：
[1] Japan Agcy Marine Earth Sci & Technol, Inst Biogeosci, Yokosuka, Kanagawa 2370061, Japan
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2012年 / 78卷 / 07期
关键词：
EXTRACELLULAR INULINASE; PURIFIED INULINASE; PURIFICATION; HYDROLYSIS;
D O I：
10.1128/AEM.07442-11
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An inulinase-producing Microbulbifer sp. strain, JAM-3301, was isolated from a deep-sea sediment. An inulin operon that contained three open reading frames was cloned and sequenced. Two of the three genes were expressed. One product was an endoinulinase, and the other was a beta-fructofuranosidase. Both enzymes worked together to effectively degrade inulin.
引用
收藏
页码：2493 / 2495
页数：3
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