Construction of a Molybdenum Disulfide-Based Colorimetric Sensor for Label-Free Infectious Disease Analysis Coupled with a Catalyzed Hairpin Assembly Reaction

被引：12
|
作者：
Dong, Yan [1 ,2 ]
Wan, Ling [1 ,2 ]
Lv, Suo [1 ,2 ]
Zhu, Dan [1 ,2 ]
Su, Shao [1 ,2 ]
Chao, Jie [1 ,2 ]
Wang, Lianhui [1 ,2 ]
机构：
[1] Nanjing Univ Posts & Telecommun, Inst Adv Mat IAM, State Key Lab Organ Elect & Informat Displays, Nanjing 210023, Peoples R China
[2] Nanjing Univ Posts & Telecommun, Inst Adv Mat IAM, Jiangsu Key Lab Biosensors, Nanjing 210023, Peoples R China
来源：
LANGMUIR | 2022年 / 38卷 / 05期
关键词：
UNMODIFIED GOLD NANOPARTICLES; HYBRIDIZATION CHAIN-REACTION; AMPLIFICATION STRATEGY; GRAPHENE OXIDE; DNA; MOS2; PROBE; PLATFORM;
D O I：
10.1021/acs.langmuir.1c02891
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The simple and accurate determination of pathogenic infectious diseases is very beneficial to public health prevention and control. For this purpose, we designed a colorimetric sensor for label-free avian influenza A (H7N9) virus gene sequence detection based on gold@platinum core-shell bimetallic-nanoparticle-decorated molybdenum disulfide (MoS2-Au@Pt) nanocomposites. MoS2-Au@Pt nanocomposites were used as nanoenzymes to catalyze 3,3',5,5'-tetramethylbenzidine (TMB) by hydrogen peroxide (H2O2) because of their intrinsic peroxidase-mimicking activity. Coupled with different affinities of MoS2-Au@Pt nanocomposites toward single-stranded (ss) and double-stranded (ds) DNA and the target-triggered catalyzed hairpin assembly (CHA) reaction, the proposed sensor can qualitatively and quantitatively determine H7N9 by the naked eye. Experimental results showed that this sensor can detect H7N9 in buffer and real samples because of its high sensitivity, selectivity, and repeatability.
引用
收藏
页码：1791 / 1796
页数：6
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