Novosphingobium ipomoeae sp nov., isolated from a water convolvulus field

A bacterial strain designated Tese-5(T) was isolated from a water convolvulus field in Taiwan and characterized using the polyphasic taxonomic approach. Strain Tese-5(T) was an aerobic, Gram-stain-negative, non-motile, rod-shaped bacterium and formed bright yellow coloured colonies. Strain Tese-5(T) grew at 15-35 degrees C (optimum, 30 degrees C), with 0-1.0% NaCl (optimum, 0-0.5 %) and at pH 5.5-7 (optimum, pH 6). The major fatty acids (> 10 %) of strain Tese-5(T) were C-18 (:) (1)omega 7c, summed feature 3 (C-16 : (1)omega 7c and/or C-16 : (1)omega 6c) and C-16 : 0. The polar lipid profile comprised phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine and sphingoglycolipid. The major polyamine was spermidine. The major isoprenoid quinone was Q-10. The DNA G+C content was 65.7 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Tese-5(T) belonged to the genus Novosphingobium and showed the highest levels of sequence similarity to Novosphingobium chloroacetimidivorans BUT-14(T) and Novosphingobium mathurense SM117(T) (96.3 %). Phenotypic characteristics of the novel strain also differed from those of the closest-related species of the genus Novosphingobium. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain Tese-5(T) represents a novel species in the genus Novosphingobium, for which the name Novosphingobium ipomoeaesp. nov. is proposed. The type strain is Tese-5(T) (= BCRC 80904(T) = LMG 28838(T) = KCTC 42656(T)).