Integration of Langerhans-like cells into a human skin equivalent

被引:21
|
作者
Laubach, Vesselina [1 ]
Zoeller, Nadja [1 ]
Rossberg, Maila [1 ]
Goerg, Kerstin [1 ]
Kippenberger, Stefan [1 ]
Bereiter-Hahn, Juergen [2 ]
Kaufmann, Roland [1 ]
Bernd, August [1 ]
机构
[1] Goethe Univ Frankfurt, Dept Dermatol Venerol & Allergol, Univ Hosp, D-60590 Frankfurt, Germany
[2] Goethe Univ Frankfurt, Kinemat Cell Res Grp, D-60590 Frankfurt, Germany
关键词
Langerhans cells; MUTZ-3 cell line; Full-thickness skin model; COLONY-STIMULATING FACTOR; HUMAN PERIPHERAL-BLOOD; TUMOR-NECROSIS-FACTOR; AIR-LIQUID INTERFACE; DENDRITIC CELLS; LINE MODEL; DIFFERENTIATION; INTERLEUKIN-4; KERATINOCYTES; ANTIGEN;
D O I
10.1007/s00403-010-1092-x
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Studies regarding cellular interactions between Langerhans cells and other skin cells are somehow hampered by the difficult cultivation of these cells in vitro. Here, we show that the human MUTZ-3 cell line can be differentiated into Langerhans-like cells in the presence of a cytokine cocktail including GM-CSF, TGF-beta 1 and TNF-alpha. We used the expression of langerin, CD1a, CCR6 and the intracellular presence of Birbeck granules to identify the differentiated MUTZ-3 cells (MUTZ-3-LCs). The aim of this study was to integrate MUTZ-3-LCs into a three-dimensional full-thickness skin model. On top of fibroblast-containing collagen matrix a mixture of primary human keratinocytes and MUTZ-3-LCs were seeded and cultured for 24 h. Subsequently, the models were lifted up to the air-liquid interface. Histological evaluation featured a fully stratified epidermis with all characteristic epidermal strata. Langerin-positive cells were detected suprabasally within the epidermis indicating that keratinocytes provide environmental conditions for long-time maintenance of MUTZ-3-LCs. These skin models provide a tool to further investigate the interactions between Langerhans-like cells and other skin cells and particularly learn more about the cutaneous immune response.
引用
收藏
页码:135 / 139
页数:5
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