Association Between Presence of Virulence Genes and Antibiotic Resistance in Clinical Klebsiella Pneumoniae Isolates

被引：36
|
作者：
Derakhshan, Safoura [1 ]
Peerayeh, Shahin Najar [1 ]
Bakhshi, Bita [1 ]
机构：
[1] Tarbiat Modares Univ, Dept Bacteriol, Fac Med Sci, Tehran, Iran
来源：
LABORATORY MEDICINE | 2016年 / 47卷 / 04期
关键词：
Klebsiella pneumoniae; antibiotic resistance; virulence genes; integrons; extended-spectrum beta-lactamase; bacterial capsule; SPECTRUM BETA-LACTAMASES; CLASS; INTEGRONS; ESCHERICHIA-COLI; PHYLOGENETIC GROUPS; STRAINS; TEHRAN; IRAN;
D O I：
10.1093/labmed/lmw030
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Objective: To investigate the presence of rmpA and wcaG virulence genes and Class 1, 2, and 3 integrons, and to evaluate a relationship between antibiotic resistance and virulence in Klebsiella pneumoniae. Methods: We collected a total of 200 K. pneumoniae isolates from hospitals in Tehran, Iran. Antibiotic susceptibility was determined using the disk diffusion method. The extended-spectrum b-lactamase (ESBL) producers were detected using the combination disk method. We detected the rmpA and wcaG genes and class 1, 2, and 3 integrons via polymerase chain reaction (PCR). The chi(2) test was used for statistical evaluation. Results: Of 200 isolates, 115 (57.5%) were ESBL producers; 74.0% carried the class 1 integron, and 1.0% carried the class 2 integron. The gene rmpA was detected in 7% of isolates and the gene wcaG in 23.5% of isolates. Integron-positive isolates showed a higher prevalence of wcaG compared with to integron-negative isolates (P<.05). Conclusion: Our results showed a correlation between presence of virulence gene and antibiotic resistance in K. pneumoniae.
引用
收藏
页码：306 / 311
页数：6
相关论文
共 50 条
  • [21] Molecular diversity of Klebsiella pneumoniae clinical isolates: antimicrobial resistance, virulence, and biofilm formation
    Kafa, Ayse Humeyra Taskin
    Aslan, Rukiye
    Dastan, Sevgi Durna
    Celik, Cem
    Hasbek, Mursit
    Eminoglu, Aysenur
    [J]. NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, 2024,
  • [22] Aminoglycoside Resistance in Clinical Klebsiella pneumoniae Isolates
    Tang, Xiaolong
    Cai, Shuyu
    Chen, Jia
    Xu, Lifa
    Wang, Jian
    Li, Wenyue
    Zhang, Xiuyun
    Sun, Lin
    Fang, Liyi
    Zhou, Xin
    Jiang, Zhenyou
    Liu, Peng
    Wei, Liuhua
    [J]. JOURNAL OF PURE AND APPLIED MICROBIOLOGY, 2013, 7 (04): : 2993 - 3000
  • [23] Fluoroquinolone Resistance in Clinical Isolates of Klebsiella Pneumoniae
    Geetha, Pacha Venkataramana
    Aishwarya, Kayanam Vijaya Lalitha
    Mariappan, Shanthi
    Sekar, Uma
    [J]. JOURNAL OF LABORATORY PHYSICIANS, 2020, 12 (02) : 121 - 125
  • [24] Bacterial Resistance in Clinical Isolates of Klebsiella Pneumoniae
    [J]. 中华医学杂志(英文版), 1995, (05) : 9 - 9
  • [25] Characteristics of antibiotic resistance mechanisms and genes of Klebsiella pneumoniae
    Li, Yanping
    Kumar, Suresh
    Zhang, Lihu
    Wu, Hongjie
    Wu, Hongyan
    [J]. OPEN MEDICINE, 2023, 18 (01):
  • [26] Characterization of Klebsiella pneumoniae complex isolates from pigs and humans in farms in Thailand: population genomic structure, antibiotic resistance and virulence genes
    Leangapichart, Thongpan
    Lunha, Kamonwan
    Jiwakanon, Jatesada
    Angkititrakul, Sunpetch
    Jarhult, Josef D.
    Magnusson, Ulf
    Sunde, Marianne
    [J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2021, 76 (08) : 2012 - 2016
  • [27] Proteome analysis, genetic characterization, and antibiotic resistance patterns of Klebsiella pneumoniae clinical isolates
    Marzouk, Eman
    Abalkhail, Adil
    Alqahtani, Jamaan
    Alsowat, Khalid
    Alanazi, Menwer
    Alzaben, Feras
    Alnasser, Abdulaziz
    Alasmari, Anas
    Rawway, Mohammed
    Draz, Abdelmaged
    Abu-Okail, Akram
    Altwijery, Abdulmohsen
    Moussa, Ihab
    Alsughayyir, Sulaiman
    Alamri, Saleh
    Althagafi, Mohammed
    Almaliki, Abdulrahman
    Elmanssury, Ahmed Elnadif
    Elbehiry, Ayman
    [J]. AMB EXPRESS, 2024, 14 (01)
  • [28] Molecular detection of virulence genes in Klebsiella Pneumoniae clinical isolates from Kurdistan Province, Iran
    Shakib, Pegah
    Kalani, Morovvat Taheri
    Ramazanzadeh, Rashid
    Ahmadi, Amjad
    Rouhi, Samaneh
    [J]. BIOMEDICAL RESEARCH AND THERAPY, 2018, 5 (08): : 2581 - 2589
  • [29] Investigation of antibiotic resistance and the presence of integron genes among ESBL producing Klebsiella isolates
    Pirzaman, Ameneh Najafgholizadeh
    Mojtahedi, Ali
    [J]. META GENE, 2019, 19 : 37 - 41
  • [30] Antibiotic pressure on the acquisition and loss of antibiotic resistance genes in Klebsiella pneumoniae
    Simner, Patricia J.
    Antar, Annukka A. R.
    Hao, Stephanie
    Gurtowski, James
    Tamma, Pranita D.
    Rock, Clare
    Opene, Belita N. A.
    Tekle, Tsigereda
    Carroll, Karen C.
    Schatz, Michael C.
    Timp, Winston
    [J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2018, 73 (07) : 1796 - 1803
12345