Zoledronic acid inhibits osteoclastogenesis in vitro and in a mouse model of inflammatory osteolysis

被引:22
|
作者
Sudhoff, H
Jung, JY
Ebmeyer, J
Faddis, BT
Hildmann, H
Chole, RA
机构
[1] Washington Univ, Sch Med, Dept Otorhinolaryngol Head & Neck Surg, St Louis, MO 63110 USA
[2] Ruhr Univ Bochum, St Elisabeth Hosp, Dept Otorhinolaryngol Head & Neck Surg, D-4630 Bochum, Germany
来源
ANNALS OF OTOLOGY RHINOLOGY AND LARYNGOLOGY | 2003年 / 112卷 / 09期
关键词
keratin; osteoclastogenesis; particle-induced osteolysis; RANKL; zoledronic acid;
D O I
10.1177/000348940311200907
中图分类号
R76 [耳鼻咽喉科学];
学科分类号
100213 ;
摘要
This study assessed effects of the bisphosphonate zoledronic acid (ZLNA) on osteoclastogenesis. To assess the effect of ZLNA on osteoclast formation in vitro, we cultured mouse bone marrow cells under conditions that promote osteoclastogenesis. Administered at concentrations from 10(-6) to 10(-9) mol/L, ZLNA led to a dose-dependent inhibition of osteoclastogenesis. Combined TUNEL staining and histochemical staining for tartrate-resistant acid phosphatase showed that ZLNA induced apoptosis in osteoclasts and monocytic precursor cells. To study the effects of ZLNA in vivo, we placed keratin particles onto the surface of the parietal bone of mice to induce localized inflammatory bone resorption. Three experimental groups received daily subcutaneous injections of ZLNA (1, 3, or 10 mug/kg body weight) from 4 days before surgery until 5 days after keratin implantation. The ZLNA significantly reduced osteoclast recruitment in a dose-dependent manner, but did not affect the degree of inflammation or the mineral apposition rate.
引用
收藏
页码:780 / 786
页数:7
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