Bioassay-guided isolation of a novel chemoattractant for Ralstonia solanacearum in tomato root exudates

被引：5
|
作者：
Hasegawa, Takuya [1 ]
Okabe, Atsushi [2 ]
Kato, Yusuke [2 ]
Ooshiro, Atsushi [3 ]
Kawaide, Hiroshi [4 ]
Natsume, Masahiro [4 ]
机构：
[1] Tokyo Univ Agr & Technol, United Grad Sch Agr Sci, Dept Biol Prod Sci, Saiwai Cho, Fuchu, Tokyo 1838509, Japan
[2] Tokyo Univ Agr & Technol, Grad Sch Agr, Dept Bioregulat & Biointeract, Saiwai Cho, Fuchu, Tokyo 1838509, Japan
[3] Okinawa Prefectural Agr Res Ctr, Itoman, Okinawa 9010336, Japan
[4] Tokyo Univ Agr & Technol, Inst Agr, Div Bioregulat & Biointeract, Saiwai Cho, Fuchu, Tokyo 1838509, Japan
来源：
JOURNAL OF GENERAL PLANT PATHOLOGY | 2018年 / 84卷 / 01期
关键词：
Ralstonia solanacearum; Bacterial wilt; Chemotaxis; Tomato; Root exudates; PSEUDOMONAS-SOLANACEARUM; RHIZOBIUM-LEGUMINOSARUM; NODULATION GENES; CHEMOTAXIS; COLONIZATION; VIRULENCE; PATHOGENICITY; FLUORESCENS; COMPONENTS; PLANTS;
D O I：
10.1007/s10327-017-0752-5
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Tomato root exudates were analyzed using a bioassay to detect the chemoattractant for Ralstonia solanacearum. An activated charcoal-adsorbed fraction of root exudates from tomato cultivar Oogata-fukuju had chemoattractant activity for R. solanacearum strain MAFF 730138. The active component, purified using a Sep-Pak C18 cartridge, an activated charcoal column, diol-modified silica gel, and NH2-modified silica gel, is a new hydrophobic attractant. The final purified fraction produced a single peak in a diol-modified silica gel HPLC analysis.
引用
收藏
页码：20 / 26
页数：7
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