The epithelial-mesenchymal transition generates cells with properties of stem cells

被引：7128

作者：

Mani, Sendurai A. ^{[1
,3
]}

Guo, Wenjun ^{[1
]}

Liao, Mai-Jing ^{[1
]}

Eaton, Elinor Ng. ^{[1
]}

Ayyanan, Ayyakkannu ^{[4
]}

Zhou, Alicia Y. ^{[1
,2
]}

Brooks, Mary ^{[1
]}

Reinhard, Ferenc ^{[1
]}

Zhang, Cheng Cheng ^{[1
]}

Shipitsin, Michail ^{[5
,6
]}

Campbell, Lauren L. ^{[5
,7
]}

Polyak, Kornelia ^{[5
,6
,7
]}

Brisken, Cathrin ^{[4
]}

Yang, Jing ^{[8
]}

Weinberg, Robert A. ^{[1
,2
]}

机构：

[1] Whitehead Inst Biomed Res, Cambridge, MA 02142 USA

[2] MIT, Dept Biol, Cambridge, MA 02139 USA

[3] Univ Texas MD Anderson Canc Ctr, Dept Mol Pathol, Houston, TX 77054 USA

[4] Swiss Inst Expt Canc Res, NCCR Mol Oncol, Ecole Polytech Fed Lausanne, CH-1066 Epalinges, Switzerland

[5] Dana Farber Canc Inst, Dept Med Oncol, Boston, MA 02115 USA

[6] Harvard Univ, Sch Med, Dept Med, Boston, MA 02115 USA

[7] Harvard Univ, Sch Med, Program Biol & Biomed Sci, Boston, MA 02115 USA

[8] Univ Calif San Diego, Sch Med, Dept Pediat & Pharmacol, La Jolla, CA 92093 USA

来源：

CELL | 2008年 / 133卷 / 04期

关键词：

D O I：

10.1016/j.cell.2008.03.027

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The epithelial-mesenchymal transition (EMT) is a key developmental program that is often activated during cancer invasion and metastasis. We here report that the induction of an EMT in immortalized human mammary epithelial cells (HMLEs) results in the acquisition of mesenchymal traits and in the expression of stem-cell markers. Furthermore, we show that those cells have an increased ability to form mammospheres, a property associated with mammary epithelial stem cells. Independent of this, stem cell-like cells isolated from HMLE cultures form mammospheres and express markers similar to those of HMLEs that have undergone an EMT. Moreover, stem-like cells isolated either from mouse or human mammary glands or mammary carcinomas express EMT markers. Finally, transformed human mammary epithelial cells that have undergone an EMT form mammospheres, soft agar colonies, and tumors more efficiently. These findings illustrate a direct link between the EMT and the gain of epithelial stem cell properties.

引用

页码：704 / 715

页数：12

共 50 条

[41] Cancer Stem Cells and Epithelial-Mesenchymal Transition: Revisiting Minimal Residual Disease
Raimondi, C.
Gianni, W.
Cortesi, E.
Gazzaniga, P.
CURRENT CANCER DRUG TARGETS, 2010, 10 (05) : 496 - 508
[42] The role of the epithelial-mesenchymal transition in the maintenance of stemness in neural and glioma stem cells
Fernandez, Nestor
Wu, Megan
Das, Sunit
CANCER RESEARCH, 2012, 72
[43] Targeting epithelial-mesenchymal transition and cancer stem cells for chemoresistant ovarian cancer
Deng, Junli
Wang, Li
Chen, Hongmin
Hao, Jingli
Ni, Jie
Chang, Lei
Duan, Wei
Graham, Peter
Li, Yong
ONCOTARGET, 2016, 7 (34) : 55771 - 55788
[44] Emerging targets in pancreatic cancer: epithelial-mesenchymal transition and cancer stem cells
Castellanos, Jason A.
Merchant, Nipun B.
Nagathihalli, Nagaraj S.
ONCOTARGETS AND THERAPY, 2013, 6 : 1261 - 1267
[45] Immune Promotion of Epithelial-mesenchymal Transition and Generation of Breast Cancer Stem Cells
Reiman, Jennifer M.
Knutson, Keith L.
Radisky, Derek C.
CANCER RESEARCH, 2010, 70 (08) : 3005 - 3008
[46] Cancer stem cells and epithelial-mesenchymal transition: Novel therapeutic targets for cancer
Ishiwata, Toshiyuki
PATHOLOGY INTERNATIONAL, 2016, 66 (11) : 601 - 608
[47] THE CHARACTERISTICS OF EPITHELIAL-MESENCHYMAL TRANSITION IN HUMAN PROSTATE CANCER STEM PROGENITOR CELLS
Ma, Zhifang
INTERNATIONAL JOURNAL OF UROLOGY, 2014, 21 : A239 - A239
[48] Transforming growth factor-β1-induced epithelial-mesenchymal transition generates ALDH-positive cells with stem cell properties in cholangiocarcinoma
Shuang, Ze-Yu
Wu, Wen-Chao
Xu, Jing
Lin, Guohe
Liu, Yong-Cheng
Lao, Xiang-Ming
Zheng, Limin
Li, Shengping
CANCER LETTERS, 2014, 354 (02) : 320 - 328
[49] Mesenchymal stem cells protect from hypoxia-induced alveolar epithelial-mesenchymal transition
Uzunhan, Yurdaguel
Bernard, Olivier
Marchant, Dominique
Dard, Nicolas
Vanneaux, Valerie
Larghero, Jerome
Gille, Thomas
Clerici, Christine
Valeyre, Dominique
Nunes, Hilario
Boncoeur, Emilie
Planes, Carole
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2016, 310 (05) : L439 - L451
[50] Role of Mesenchymal Stem Cells in the colorectal cancer cell environment: stimulation of Epithelial-Mesenchymal Transition
Hogan, N. M.
Joyce, M. R.
Dwyer, R. M.
Kerin, M. J.
IRISH JOURNAL OF MEDICAL SCIENCE, 2013, 182 : S345 - S345

← 1 2 3 4 5 →