Production of Retroviral Vectors: Review

被引：0

作者：

Coroadinha, Ana S. ^{[1
]}

Gama-Norton, Leonor ^{[1
,2
]}

Amaral, Ana I. ^{[1
]}

Hauser, Hansjorg ^{[2
]}

Alves, Paula M. ^{[1
]}

Cruz, Pedro E. ^{[1
,3
]}

机构：

[1] ITQB UNL IBET, P-2780901 Oeiras, Portugal

[2] Helmholtz Ctr Infect Res, D-38124 Braunschweig, Germany

[3] ECBio, P-2781901 Oeiras, Portugal

来源：

CURRENT GENE THERAPY | 2010年 / 10卷 / 06期

关键词：

Packaging cells; production; purification; retrovirus; PACKAGING CELL-LINE; MURINE LEUKEMIA-VIRUS; SEVERE COMBINED IMMUNODEFICIENCY; REPLICATION-COMPETENT RETROVIRUS; CHRONIC GRANULOMATOUS-DISEASE; DEFINED CHROMOSOMAL LOCI; SINGLE-CHAIN ANTIBODIES; VIVO GENE DELIVERY; SERUM-FREE MEDIA; HIGH-TITER;

D O I：

暂无

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Retroviral vectors are presently amongst the most widely used vectors in gene therapy clinical trials to target pathologies of different origins, such as cancers, genetic diseases or neurological disorders. This review provides an overview on the evolution of retroviral vector design and production for gene therapy applications, including state of the art developments in flexible producer cells and safe vectors. In addition, production and purification processes will be addressed, with a particular focus on the improvements undertaken to increase vector productivity and to reduce the rapid loss of infectivity, which presently represent the main challenges in retroviral vectors production for gene therapy.

引用

页码：456 / 473

页数：18

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