Study of Genetic Stability of Human Bone Marrow Multipotent Mesenchymal Stromal Cells

被引：16
|
作者：
Nikitina, V. A. [1 ]
Osipova, E. Yu. [2 ]
Katosova, L. D. [1 ]
Rumyantsev, S. A. [2 ]
Skorobogatova, E. V. [2 ]
Shamanskaya, T. V. [2 ]
Bochkov, N. P. [1 ]
机构：
[1] Russian Acad Med Sci, Med Genet Res Ctr, Moscow, Russia
[2] Russian Minist Hlth & Social Dev, Fed Ctr Pediat Hematol Oncol & Immunol, Moscow, Russia
来源：
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2011年 / 150卷 / 05期
基金：
俄罗斯基础研究基金会;
关键词：
multipotent mesenchymal stromal cells; karyotype; aneuploidy; FISH; ACUTE MYELOID-LEUKEMIA; STEM-CELL; TRISOMY-8; THERAPY;
D O I：
10.1007/s10517-011-1207-1
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Immunophenotype, proliferation rate, and genetic stability parameters of bone marrow multipotent mesenchymal stromal cells were studied. Despite the reduction of proliferative activity by passages 11-12, the cells retained the characteristic immunophenotype. The incidence of spontaneous aneuploidy for autosomes 6, 8, 11 and sex chromosomes was evaluated. Two cultures of mesenchymal stromal cells carrying aneuploid cell clones were detected: with chromosome 8 trisomy and X chromosome monosomy. The results indicate the possibility of genetic transformation and selection of mesenchymal stromal cells with abnormal karyotype during in vitro culturing.
引用
收藏
页码：627 / 631
页数：5
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