Image-based high-throughput quantification of cellular fat accumulation

被引：20
|
作者：
Dragunow, Mike [1 ]
Cameron, Rachel
Narayan, Pritika
O'Carroll, Simon
机构：
[1] Univ Auckland, Fac Med & Hlth Sci, Dept Pharmacol, Auckland, New Zealand
[2] Univ Auckland, Fac Med & Hlth Sci, Dept Anat, Auckland, New Zealand
[3] Univ Auckland, Fac Med & Hlth Sci, Natl Res Ctr Growth & Dev, Auckland, New Zealand
来源：
JOURNAL OF BIOMOLECULAR SCREENING | 2007年 / 12卷 / 07期
关键词：
image analysis; lipids; high-content screening;
D O I：
10.1177/1087057107306502
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A number of biochemical methods are available for measuring fat accumulation in cell culture. The authors report a simple image-based method for measuring fat accumulation in adipocytes using a combination of high-throughput brightfield microscopy and image analysis, which was validated biochemically using Oil-Red-O. The quickest and most accurate method of analysis was one based on thresholding brightfield images and determining the area of fat droplets per image. Thus, the authors have developed a simple high-throughput, label-free method for measuring fat accumulation that is applicable to any cell or tissue type where fat droplets are visible under light microscopy.
引用
收藏
页码：999 / 1005
页数：7
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