Transferability of sponge gourd EST-SSR markers for genetic diversity assessment of Luffa species

被引:1
|
作者
Bhutia, Nangsol D. [1 ]
Sureja, A. K. [1 ]
Arya, Lalit [1 ,2 ]
Munshi, A. D. [1 ]
Verma, Manjusha [1 ]
机构
[1] ICAR Indian Agr Res Inst, Div Vegetable Sci, New Delhi 110012, India
[2] ICAR Natl Bur Plant Genet Resources, New Delhi 110012, India
关键词
EST-SSR; Luffa acutangula; Lufa hermaphrodita;
D O I
10.5958/0974-0112.2017.00119.0
中图分类号
S6 [园艺];
学科分类号
0902 ;
摘要
Genetic diversity was studied in 47 Luffa genotypes with 17 EST-SSR primers, which generated 34 alleles, ranging 1-2 loci per primer. Forty seven genotypes were broadly classified into two different clusters. Cluster I comprised of genotypes DRG-98, Utkal Tripti, DRG-6, Sel-102, Pusa Nasdar, Pusa Nutan, Arka Sujat, DRG-73, DRG-61, DRG-42 and DRG-50, while Cluster II consisted of 36 genotypes, respectively. EST-SSR primer C90830_ G3 was found to be highly informative with PIC value of 0.3750. The variability in the species could be credited to introgression and selection as a result of long history of cultivation under varied climatic conditions. The present data provide adequate evidence of the applicability of EST-SSR markers for diversity analyses, cultivar identification and characterization of the Luffa germplasm.
引用
收藏
页码:615 / 617
页数:3
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