The FHIT gene at 3p14.2 is abnormal in lung cancer

被引：535

作者：

Sozzi, G ^{[1
]}

Veronese, ML ^{[1
]}

Negrini, M ^{[1
]}

Baffa, R ^{[1
]}

Cotticelli, MG ^{[1
]}

Inoue, H ^{[1
]}

Tornielli, S ^{[1
]}

Pilotti, S ^{[1
]}

DeGregorio, L ^{[1
]}

Pastorino, U ^{[1
]}

Pierotti, MA ^{[1
]}

Ohta, M ^{[1
]}

Huebner, K ^{[1
]}

Croce, CM ^{[1
]}

机构：

[1] IST NAZL TUMORI,I-20133 MILAN,ITALY

来源：

CELL | 1996年 / 85卷 / 01期

关键词：

D O I：

10.1016/S0092-8674(00)81078-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To determine the role of the FHIT gene, which encompasses the fragile site at 3p14.2, we analyzed 59 tumors of the small cell and non-small cell type by reverse transcription of FHIT mRNA, followed by PCR amplification and sequencing of products. Allelic losses affecting the gene were evaluated by microsatellite polymorphism analysis and genomic alterations by hybridization using cDNA and genomic probes. Small cell lung tumors (80%) and non-small cell lung cancers (40%) showed abnormalities in RNA transcripts of FHIT, and 76% of the tumors exhibited loss of FHIT alleles. Abnormal lung tumor transcripts lack two or more exons of the FHIT gene. Small cell lung cancer tumors and cell lines were analyzed by Southern blotting and showed rearranged BamHI fragments. These data suggest a critical role of the FHIT gene in lung carcinogenesis.

引用

页码：17 / 26

页数：10

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