Parvibaculum indicum sp. nov., isolated from deep-sea water

A taxonomic study was carried out on strain P31(T), which was isolated from a polycyclic aromatic hydrocarbon (PAH)-degrading consortium enriched with deep-sea water of the Indian Ocean. The isolate was Gram-reaction-negative, rod-shaped, motile by means of a polar flagellum and incapable of reducing nitrate to nitrite. Growth was observed at 0.5-8% NaCl and at 10-41 degrees C. Strain P31(T) was unable to degrade Tween 80 or gelatin. The major respiratory quinone was ubiquinone 11 (Q-11). The dominant fatty acids were C-18:1 omega 7c(39.79%), 11-methyl C-18:1 omega 7c (17.84%), C-19:0 cycloc omega 8c (12.05%) and C-18:0 (6.09%). The G + C content of the chromosomal DNA was 62.1 mol%. A phylogenetic tree based on 16S rRNA gene sequence analysis showed that strain P31(T) and Parvibaculum lavamentivorans DS-1(T) formed a distinct lineage in the family Phyllobacteriaceae; these two strains showed 95.7 % sequence similarity, while similarities between P31(T) and other members of the genus Parvibaculum were below 93%. Based on the genotypic and phenotypic data, strain P31(T) represents a novel species of the genus Parvibaculum, for which the name Parvibaculum indicum sp. nov. is proposed. The type strain is P31(T) (=CCTCC AB 208230(T) =LMG 24712(T) =MCCC 1A01132(T)).